| 低温胁迫对裂殖壶菌DHA生物合成及SOD表达的影响 |
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| 引用本文： | 刘静,高媛媛,江贤章,毛若雨,田宝玉,柯崇榕,吴松刚,黄建忠. 低温胁迫对裂殖壶菌DHA生物合成及SOD表达的影响[J]. 药物生物技术, 2010, 0(1) |
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| 作者姓名： | 刘静 高媛媛 江贤章 毛若雨 田宝玉 柯崇榕 吴松刚 黄建忠 |
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| 作者单位： | 福建师范大学工业微生物教育部工程研究中心;生命科学学院;福建省现代发酵技术工程研究中心; |
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| 基金项目： | 国家自然科学基金(30370028); 福建省自然科学基金重大项目(2003F005); 福建省自然科学基金(2008F3036); 福建省自然科学基金青年创新项目(2009J05057); 福建省科技平台(2005Q007) |
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| 摘 要： | 培养温度对裂殖壶菌产油脂中二十二碳六烯酸(Docosahexaenoic acid,DHA)含量以及超氧化物歧化酶(Superoxide dismutase,SOD)表达水平均有重大影响。细胞干重测定和气相质谱联用法检测了不同温度下裂殖壶菌Schizochytrium sp.FJU-512的生长和脂肪酸组成,荧光定量PCR法比较各温度下SOD mRNA表达量。结果表明,低温有利于DHA的合成,低温胁迫下SOD mRNA表达量最高。探讨裂殖壶菌耐低温胁迫的机制可能是低温增加培养基中溶解氧水平,氧化应激造成SOD的高表达,加快活性氧自由基的清除,促进DHA等不饱和脂肪酸的积累,增强膜的流动性以适应低温环境。其次,从DHA高产菌株裂殖壶菌Schizochytrium sp.FJU-512 EST文库筛选出SOD基因,并应用生物信息学分析该氨基酸序列。
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| 关 键 词： | 超氧化物歧化酶 低温 裂殖壶菌 二十二碳六烯酸 |
Effects on Docosahexaenoic Acid Biosynthesis and Expression of Superoxide Dismutase in Schizochytrium at Low Temperature |
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| LIU Jing,GAO Yuan-yuan,JIANG Xian-zhang,MAO Ruo-yu,TIAN Bao-yu,KE Chong-rong,WU Song-gang,HUANG Jian-zhong. Effects on Docosahexaenoic Acid Biosynthesis and Expression of Superoxide Dismutase in Schizochytrium at Low Temperature[J]. Pharmaceutical Biotechnology, 2010, 0(1) |
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| Authors: | LIU Jing GAO Yuan-yuan JIANG Xian-zhang MAO Ruo-yu TIAN Bao-yu KE Chong-rong WU Song-gang HUANG Jian-zhong |
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| Affiliation: | LIU Jing,GAO Yuan-yuan,JIANG Xian-zhang,MAO Ruo-yu,TIAN Bao-yu,KE Chong-rong,WU Song-gang,HUANG Jian-zhong(Engineering Research Center of Industrial Microbiology,Ministry of Education,Fujian Normal University,College of Life Sciences,Engineering Research Center of Fujian Modern Fermentation Technology,Fuzhou 350108,China) |
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| Abstract: | The cultural temperatures had much effect on docosahexaenoic acid (DHA) content in total lipid extracted from dried cell with different superoxide dismutase (SOD) expression levels. The growth and fatty acid composition of Schizochytrium sp. FJU-512 at different cultural temperatures were determined by dried cell and GC-MS. The expression level of SOD mRNA at different temperatures was compared by fluorescence quantitative-PCR. The results showed that low temperature was beneficial to DHA biosynthesis in ma... |
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| Keywords: | Superoxide dismutase Low temperature Schizochytrium Docosahexaenoic acid |
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