| HPLC法测定脑脉泰胶囊中银杏总黄酮的含量 |
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| 引用本文： | 陈薇,唐春燕,韦鑫.HPLC法测定脑脉泰胶囊中银杏总黄酮的含量[J].中国药师,2009,12(2). |
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| 作者姓名： | 陈薇 唐春燕 韦鑫 |
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| 作者单位： | 1. 桂林医学院附属医院药剂科,广西桂林,541001
2. 桂林医学院2008届毕业生 |
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| 摘 要： | 目的:建立高效液相色谱法测定脑脉泰胶囊中银杏总黄酮含量的方法。方法:色谱柱:伊利特-C_(18)柱(250mm×4.6 mm,5μm),流动相:乙腈-甲醇-水(9:39:52),磷酸调节pH至4.0,流速:0.8ml·min~(-1),检测波长:370nm,柱温:35℃。结果:槲皮素、山奈素、异鼠李素检测浓度的线性范围分别为4.20～25.20(r=0.999 7),4.85～29.10(r=0.999 5),2.02～12.12μg·ml~(-1)(r=0.999 2);平均回收率分别为99.90%,100.10%,98.33%,RSD分别为0.63%,0.80%,0.99%。结论:本法快速、简便、准确、重复性好,可用于脑脉泰胶囊的质量控制。
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| 关 键 词： | 高效液相色谱法 脑脉泰胶囊 银杏总黄酮 含量测定 |
Determination of Ginkgo Biloba Flavonoids in Naomaitai Capsule by HPLC |
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| Chen Wei,Tang Chunyan,Wei Xin.Determination of Ginkgo Biloba Flavonoids in Naomaitai Capsule by HPLC[J].China Pharmacist,2009,12(2). |
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| Authors: | Chen Wei Tang Chunyan Wei Xin |
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| Institution: | Chen Wei; Tang Chunyan(Dept.of Pharmacy; Affiliated Hospital of Guilin Medical College; Guilin 541001 China) Wei Xin(Guilin Medical College 2008 Session Graduates); |
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| Abstract: | Objective:To establish an HPLC method for the determination of ginkgo biloba flavonoids in Naomaitai capsules. Method:The determination was performed on the Elite C_(18) column(250mm×4.6mm,5μm) with column temperature at 35℃.The mobile phase consisted of acetonitrile-methanol-water(9:39:52)(adjust pH to 4.0 with phosphoric acid),the flow rate was 0.8ml? min~(-1),the detection wavelength was at 370 nm.Result:The linear ranges of quercetin,isorhamnetin and kaempferol were 4.20～25. 20μg·ml~(-1)(r=0.999 7),4.85... |
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| Keywords: | HPLC Naomaitai capsule Ginkgo biloba flavonoids Content determination |
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