维、汉妇女宫颈癌患者感染HPV亚型和TERC基因扩增的关系

目的探讨新疆地区维吾尔族、汉族妇女宫颈癌感染人乳头瘤病毒（HPV）不同亚型及人染色体端粒酶基因（TERC）扩增差异。方法采用导流杂交基因芯片技术检测维吾尔族和汉族各200例宫颈癌患者的21种HPV亚型感染的分布情况。采用荧光原位杂交技术（FISH）检测23例维吾尔族和22例汉族宫颈癌患者的TERC基因扩增的情况。结果①维、汉族宫颈癌的HPV感染谱不同。维吾尔族宫颈癌中HPV高危亚型感染排序由高到低主要是：HPV16、58、18、52和31等亚型;而汉族宫颈癌的排序是：HPV16、31、58、18和52等亚型。②维吾尔族宫颈癌中HPV亚型多重感染者为43例,占21.83%;汉族宫颈癌组织中多重感染者为27例,占13.78%（P〈0.05）。③维、汉族宫颈癌TERC基因平均扩增倍数,在HPV各亚型感染组间差异无统计学意义,但在各族患者中HPV多重感染和单一感染其差异均有统计学意义（P〈0.05）。结论维吾尔族宫颈癌患者HPV高危亚型多重感染较汉族高;在各族患者中多重HPV高危亚型感染较单一亚型感染者有更多TERC基因扩增。

Study on genotypes of human papilloma virus infected Uigur and Han women with cervical cancer and their relationships with TERC gene amplification

Objective To explore the difference of genotypes of human papilloma virus（HPV） infected Uigur and Han women with cervical cancer and the difference of TERC gene amplification.Methods Twenty-one HPV subtype infection was detected by channelization hybridization gene chip in 200 cases of Uigur and 200 cases of Han cervical cancer.TERC amplification was tested by fluorescence in situ hybridization（FISH） in 23 cases of Uigur and 22 cases of Han patients.Results ① The HPV infection spectrums of Uygur,Han women with cervical cancer were different.The most common subtypes of high-risk HPV infected Uigur women with cervical cancer were HPV16,HPV58,HPV18,HPV52 and HPV31 sequently;while in Han were HPV16,HPV31,HPV58,HPV18 and HPV52.② More multiple infection was found in Uigur group than in Han group（43 vs 27 women,21.83% vs.13.78%,P〈0.05） ③ No difference was found in the TERC gene amplification among the infectioned HPV subtypes.But statistically more TERC gene amplification was detected in the multi-type infection group than and single-type infection.Conclusions More multi-type high-risk HPV infection is found in Uigur than Han patients.The multi-type high-risk HPV infection is associated to TERC gene amplification in both Uigur and Han women.