丹酚酸B预处理内皮祖细胞在大鼠骨髓间充质干细胞分化过程中对心肌早期基因表达的影响

目的 探讨丹酚酸B预处理内皮祖细胞(EPCs)联合大鼠骨髓间充质干细胞（BMSc）移植于梗死心肌对BMSc分化过程中早期基因Nkx2-5、GATA-4 mRNA表达的影响, 从而找出能够使两种细胞达到最佳协同作用的移植方法。

Effects of Salvianolic Acid B Preconditioning Endothelial Progenitor Cells on Expressions of Myocardial Genes in Bone Mesenchymal Stem Cells at the Early Cell Differentiation Stage of Rats

Objective To investigate the effects of Salvianolic acid B preconditioned endothelial progenitor cells （EPCs） on the Nkx2.5 and GATA-4 gene expressions at the early stage of cell differentiation of bone mesenchymal stem cells （BMSc） transplanted into infarcted myocardium, in order to find out the best synergism for co-transplantation of the two kinds of cells. Methods BMSc and EPCs of rats were isolated and cultured, and rats were modeled into acute myocardial infarction （AMI） by left coronary artery ligation. Then the EPCs preconditioned with different concentrations of Salvianolic acid B and BMSc or DMEM medium were implanted into heart ischemia area. Expressions of Nkx2.5 and GATA-4 mRNA expressions in myocardium were detected by Realtime RT-PCR 4 weeks later. Results Compared with those in the non-implanted model rats＇ myocardium, the gene expression of Nkx2.5 and GATA-4 mRNA were significantly higher in all the transplantation receptive groups, comparisons between the implanted groups showed that the highest value of expressions （2. 654 ± 0. 606 of Nkx2.5 and 1. 573 ±0. 372 of GATA-4） displayed in the group contained more EPCs, for 8-fold to BMSc in volume. Conclusion BMSc can differentiate into cardiac muscle like cells, and condition of their differentiation is related with the degree of the internal environment improved.