白介素-1对培养人表皮黑素细胞增殖及黑素合成的影响研究

目的:建立正常人表皮黑素细胞体外培养体系,观察不同浓度的白介素-1α,白介素-1β对体外培养正常人黑素细胞的细胞增殖及黑素合成的影响。方法:采用四甲基偶氮唑盐(MTT)法测定白介素-1α及白介素-1β对黑素细胞增值的影响,NaOH裂解法测定黑素生成量,流式细胞仪检测黑素细胞的凋亡率。结果:白介素-1α及白介素-1β对黑素细胞活力有抑制作用,能使细胞增殖能力降低,黑素合成减少,增加黑素细胞的凋亡率。结论:白介素-1α及白介素-1β对体外培养的黑素细胞增殖及黑素生成都有抑制作用,这为临床应用白介素-1α及白介素-1β治疗色素性疾病提供了实验依据。

Study on effects of Interleutin-1 on cell proliferation and melanin synthesis in cultured human melanocytes

Objective Establishing the cultured system of human normal melanocytes in vitro,and studying effects of IL-1α and IL-1β on cultured human melanocytes.Methods MTT method,NaOH assay were employed to measure the rate of proliferation,melanin synthesis of melanocytes respectively;apoptosis rates of melanocytes were detected by using flow cytometer.Results Not only IL-1α but also IL-1β showed a significant decrease of the rate of melanocyte proliferation in concentration and time-dependent manner,inhibited melanin synthesis and increased melanocyte apoptosis.Conclusion It suggests that not only IL-1α but also IL-1β at the concentration of 50-200ng/ml is able to suppress melanocytic proliferation and melanin synthesis.It may be developed to be a potential skin lightening agent and worth to study later.