| Effect of pretreatment with different concertrations of morphine, fentanyl and tramadol on the differentiation of human helper T cells in vitro |
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| 引用本文： | Yanning Qian,Wenjie Jin,Ping Chen,Ling Wang,Huijuan Wang,Yuke Tian,Xuejun Zhou. Effect of pretreatment with different concertrations of morphine, fentanyl and tramadol on the differentiation of human helper T cells in vitro[J]. 南京医科大学学报(自然科学版), 2005, 19(6): 313-315 |
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| 作者姓名： | Yanning Qian Wenjie Jin Ping Chen Ling Wang Huijuan Wang Yuke Tian Xuejun Zhou |
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| 作者单位： | [1]Department of Anesthesiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China [2]Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| 摘 要： | Objective： To investigate and compare the .effects of different concentrations of morphine, fentanyl and tramadol on the differentiation of human adult helper T cells in vitro. Methods： Twenty out-patients without immune disease were selected and their peripheral blood was collected. Then the Whole blood of peripheral blood mononuclear cells （PBMCs） were pretreated with different concentration of morphine, fentanyl and tramadol for 24 h. The level of CD4^＋ IFN-γ^＋ IL-2^＋/CD4^＋ IL-4^＋ IL-10^＋ was analyzed by three-color flow cytometry, and the CD4^＋ CCR5^＋ and CD4^＋ CCR3 ^＋ cells were counted to observe the imbalance of Th2/Th2. Results： The number of Th2 increased significantly and the ratio of Th2/Th2 decreased dramatically compared with the control group, and there was a dose-dependent fashion in all drugs. Conclusion： Morphine, fentanyl and tramadol can direct Th0 cells toward Th2 differentiation, especially morphine and fentanyl.
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| 关 键 词： | 预处理 吗啡 芬太奴 T细胞 药品管理 止痛药品 |
| 收稿时间： | 2005-07-16 |
Effect of pretreatment with different concertrations of morphine, fentanyl and tramadol on the differentiation of human helper T cells in vitro |
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| Yanning Qian,Wenjie Jin,Ping Chen,Ling Wang,Huijuan Wang,Yuke Tian,Xuejun Zhou. Effect of pretreatment with different concertrations of morphine, fentanyl and tramadol on the differentiation of human helper T cells in vitro[J]. Acta Universitatis Medicinalis Nanjing, 2005, 19(6): 313-315 |
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| Authors: | Yanning Qian Wenjie Jin Ping Chen Ling Wang Huijuan Wang Yuke Tian Xuejun Zhou |
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| Affiliation: | 1. Department of Anesthesiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
2. Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Objective: To investigate and compare theeffects of different concentrations of morphine, fentanyl and tramadol on the differentiation of human adult helper T cells in vitro. Methods: Twenty out-patients without immune disease were selected and their peripheral blood was collected. Then the whole blood of peripheral blood mononuclear cells (PBMCs) were pretreated with different concentration of morphine, fentanyl and tramadol for 24 h. The level of CD4+ IFN-γ+ IL-2+/CD4+ IL-4+ IL-10+ was analyzed by three-color flow cytometry, and the CD4 + CCR5 + and CD4 + CCR3 + cells were counted to observe the imbalance of Th2/Th2. Results:The number of Th2 increased significantly and the ratio of Th2/Th2 decreased dramatically compared with the control group, and there was a dose-dependent fashion in all drugs. Conclusion: Morphine, fentanyl and tramadol can direct Th0 cells toward Th2 differentiation,especially morphine and fentanyl. |
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| Keywords: | morphine fentanyl tramadol helper T cells cytokines |
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