Metabolomic characteristics of spontaneously hypertensive rats under chronic stress and the treatment effect of Danzhi Xiaoyao Powder,a traditional Chinese medicine formula |
| |
| Authors: | Shuai Chen Jin Hu Deng-cheng Lu Hong-yi Liu Shan-shan Wei |
| |
| Institution: | 1. College of Basic Medical Sciences, Yunnan University of Chinese Medicine, Kunming 650500, Yunnan Province, China;2. Department of Endocrinology, Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming 650021, Yunnan Province, China;3. Key Laboratory of Syndrome Micro-Differentiation of Yunnan Province, Kunming 650500, Yunnan Province, China;1. Department of Radiology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;2. Department of Acupuncture & Moxibustion, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;1. Department of Biomedical Engineering, Southwest State University, Kursk 305040, Russian Federation;2. Electrical Energy Department, Balqa Applied University, Amman 11937, Jordan;3. Civil Engineering Department, Zarqa University, Zarqa Governorate 13222, Jordan;4. Saint-Petersburg National Research University of Information Technologies, Mechanics and Optics, Saint-Petersburg 197101, Russian Federation;5. Pediatric Faculty, Kursk State Medical University, Kursk 305041, Russian Federation;1. Andalusian School of Public Health, 18011 Granada, Spain;2. Unit of Clinical Management of Preventive Medicine and Public Health and Occupational Medicine, Puerta del Mar University Hospital, 11009 Cádiz, Spain;3. Consortium for Networked Biomedical Research in Epidemiology and Public Health, 28029 Madrid, Spain;4. Institute for Biosanitary Research (ibs.GRANADA), 18012 Granada, Spain;1. Department of General Practice, Changhai Hospital, Naval Medical University, Shanghai 200433, China;2. Department of General Practice, Changhai Hongkou Hospital, Shanghai 200081, China;3. Department of Special Clinic, Changhai Hospital, Naval Medical University, Shanghai 200433, China;4. Shanghai Institute of Biochemical and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China;5. Department of Nursing, Shidong Hospital, University of Shanghai for Science and Technology, Shanghai 200438, China;6. Department of Respiratory and Critical Care Medicine, Changhai Hospital, Naval Medical University, Shanghai 200433, China;1. Gastroenterology and Hepatology Research Center, Kerman University of Medical Sciences, Kerman 7716913555, Iran;2. Department of Traditional Medicine, School of Persian Medicine, Kerman University of Medical Sciences, Kerman 7716913555, Iran;3. Student Research Committee, Afzalipour Faculty of Medicine, Kerman University of Medical Sciences, Kerman 7716913555, Iran;4. Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman 7716913555, Iran;5. Department of Community Health Nursing, Razi Faculty of Nursing and Midwifery, Kerman University of Medical Sciences, Kerman 7716913555, Iran;1. Department of Cardiology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China;2. Haematopoiesis and Leukocyte Biology Laboratory, Baker Heart and Diabetes Research Institute, Melbourne, VIC 3004, Australia |
| |
| Abstract: | ObjectiveNumerous studies have demonstrated the close relationship between chronic stress and blood pressure (BP). Hypertensive subjects exhibit exaggerated reactions to stress, especially higher BP. The mechanisms by which stress affects pre-existing hypertension still need to be explored. Danzhi Xiaoyao Powder (DP), a historical traditional Chinese medicine formula, is a promising treatment for BP control in hypertensive patients under stress. The present study investigated the metabolomic disruption caused by chronic stress and the treatment effect and mechanism of DP.MethodsSpontaneously hypertensive rats (SHRs) were subjected to chronic restraint stress (CRS) for 4 weeks. BP was measured via the tail-cuff method, and anxiety-like behavior was quantified using the elevated-plus-maze test. Meanwhile, DP was administered intragastrically, and its effects were observed. Global metabolomic analysis was performed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, followed by multivariate statistical analysis to detect differential metabolites and pathways.ResultsDP alleviated the CRS-induced increase in BP and anxiety-like behavior. Systematic metabolic differences were found among the three study groups. A total of 29 differential plasma metabolites were identified in both positive- and negative-ion modes. These metabolites were involved in triglyceride metabolism, amino acid (phenylalanine, tryptophan, and glycine) metabolism, and steroid hormone pathways.ConclusionThese findings expose the metabolomic disturbances induced by chronic stress in SHRs and suggest an innovative treatment for this disorder. |
| |
| Keywords: | |
| 本文献已被 维普 ScienceDirect 等数据库收录！ |
|
