健心平律丸对大鼠心肌缺血再灌注心律失常和水通道蛋白4的影响

目的探讨健心平律丸对大鼠心肌缺血再灌注心律失常和水通道蛋白4(aquaporin-4，AQP4)的作用。方法应用阻断左冠状动脉造成大鼠心肌缺血再灌注损伤模型。应用心电图Ⅱ导联记录、HE染色和AQP4免疫组化染色方法，观察健心平律丸对心肌缺血再灌注心律失常、死亡率、AQP4的影响。结果健心平律丸可明显减少再灌注心律失常发生率及死亡率，有减轻心肌缺血水肿损伤，增强心肌组织AQP4表达的作用。结论健心平律丸具有抗心肌缺血再灌注心律失常的作用，可能与其上调缺血心肌AQP4表达水平，减轻细胞内水肿有关。

Effect of Jianxin Pinglu Pill on Arrhythmia and Aquaporin 4 Expression in Rats with Myocardial Ischemia/Reperfusion Injury

OBJECTIVE: To explore the effect of jianxin pinglu pill (JPP) on arrhythmia and aquaporin 4 (AQP4) in rats with myocardial ischemia/reperfusion (I/R) injury. METHODS: The effects of JPP on arrhythmia, mortality and AQP4 on I/R injured rats model induced by blocking left coronary artery were observed using II lead of ECG, HE stain and AQP4 immunohistochemical stain. RESULTS: JPP showed significant effect in lowering the arrhythmia occurrence and mortality, reducing myocardial ischemic edema and injury, strengthening AQP4 expression in myocardial tissue. CONCLUSION: JPP has the effect of preventing I/R induced arrhythmia, it might be related with its action in up-regulating AQP4 expression level in myocardium and reducing the intracellular edema.