乌司他丁对大鼠肾脏机械性损伤后肾脏HSP70、IL-6表达的早期影响

目的探讨乌司他丁在大鼠肾脏机械性损伤后对肾脏组织IL-6和HSP70表达的影响及意义。方法66只Wistar大鼠,随机分为非创伤对照组、单纯创伤组、创伤后给予乌司他丁组。采用自由落体生物撞击仪撞击大鼠脊肋角复制创伤动物模型。用组织芯片技术及免疫组织化学方法检测各组创伤后1h、6h、12h、24h、36h肾脏组织中IL-6和HSP70蛋白的表达。结果对照组肾脏组织中IL-6和HSP70蛋白均呈弱阳性表达。与对照组比较,单纯创伤组在创伤后1、6、12、24h点IL-6表达增强(P<0.05),6h点HSP70表达增强(P<0.05)。创伤后注射乌司他丁组各时相点肾组织中IL-6表达明显低于单纯创伤组(P<0.05),HSP70表达在1h、24h、36h点明显高于单纯创伤组(以1h点最为显著,P<0.05)。结论乌司他丁可能通过抑制IL-6的过度表达,上调HSP70的表达,改变两者表达的比例,在创伤早期(以1h点为著)对大鼠肾脏起保护性作用。

Ulinastatin's Early Effects on Kidney's Expression of HSP70 and IL-6 after Mechanical Injury of Kidney

Objective To investigate the significance of ulinastatin's effects on kidney's expression of IL-6,HSP70 after mechanical injury.Methods Sixity-six adult female Wistar rats were randomly divided into three groups:6 in the group of control,30 in the group of trauma,30 in the group injected ulinastatin after trauma.The model of trauma rats was made with free-fall bio-impactor striking the spinal column and ribs of rats.To detect the IL-6 and HSP70 expression of each group's renal tissue at 1 h,6 h,12 h,24 h and 36 h after trauma with tissue chip and immunohistochemical stain.Results IL-6 and HSP70 expression of renal tissue was low with group of control.Compared with group of control,the expression of IL-6 strengthened at 1 h,6 h,12 h,24 h after trauma(P<0.05),the expression of HSP70 with group of trauma strengthened at 6 h after trauma(P<0.05).The expression of IL-6 with group injected ulinastatin after trauma were more lower than group of trauma,the expression of HSP70 at 1 h,24 h,36 h after trauma was higher than group of trauma especially at 1 h(P<0.05).Conclusion Ulinastatin can reduce the risk of renal injury in early times after trauma especially at 1 h.The protective effects of ulinastatin may be associated with the restrict the expression of IL-6,up-regulation of HSP70 expression,and change the proportion between IL-6 and HSP70 expression.