马桑内酯致痫大鼠大脑皮层,海马谷氨酸、门冬氨酸、甘氨酸及γ-氨基丁酸含量的测定

为了探讨氨基酸类神经递质在癫痫发病中的作用，我们采用高效液相色谱仪（HPLC）结合紫外分光光度仪分析检测了马桑内脂（CL）致病大鼠大脑皮层、海马Glu、Asp、Gly、GABA的含量变化。结果显示：CL致痫组与正常对照组相比，Glu、Asp、Gly含量增加（P<0.01，P<0.05）；GABA含量减少，但差异无显著性（P＞0．05）。这些结果提示兴奋性和抑制性氨基酸在癫痫发病中具有重要作用，可能参与了惊厥的发生、发展和惊厥后神经元损伤。

Alterations of glutamate (Glu), aspartate (Asp), glycine (Gly) and gamma aminobutyric acid (GABA) contents in cerebral cortex and hippocampus of seizure rats induced by coriaria lactone (GL)

objective: The experiment was designed to elucidate the role of excitatory and inhibitoryamino acids (EAAs, IAAs) in the pathogenesis of epilepsy. Methods: We determined the contents of Glu,Asp.Gly and GABA in cerebral cortex and hippocampus in rats 60 minutes post-seizure induced by CL byhigh performance liquid chromatography with fluorimetric detection of OPT derivatives. ` Results: Theresults showed that the levels of Glu' Asp and Gly at two regions in seizwe rats induced by CL wereincreased (P<0. 01, P<0. 05 ), and there was no difference in GABA content between convulsive andnormal control groups (P > 0.05). Conclusion: These findings indicate that EAAs and IAAs areprobabaly involved in the pathophysiology of epileptogenesis, neuronal damage and death after seizure.