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Determination of picogram levels of brain serotonin by a simplified liquid chromatographic electrochemical detection assay |
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| Authors: | Jerry J Warsh Andrew Chiu Damodar D Godse Donald V Coscina |
| Institution: | Section of Neurochemistry Clarke Institute of Psychiatry, University of Toronto, 250 College Street, Toronto, Ontario, Canada M5T 1R8;Section of Biopsychology Clarke Institute of Psychiatry, University of Toronto, 250 College Street, Toronto, Ontario, Canada M5T 1R8 |
| Abstract: | A simplified assay is described for measurement of picogram amounts of serotonin (5-HT) by high pressure liquid chromatography with electrochemical detection (LCEC). The procedure involves pre-purification of brain 5-HT by adsorption on Amberlite CG-50. Serotonin is subsequently resolved and detected by LCEC on Zipax SCX resin. The present method gives working sensitivities of at least 100 pg, tissue recoveries of 95% and very low interassay variability (coefficientofvariation=3%). Determination of rat brain area 5-HT by this LCEC method is described and compared to other high sensitivity methods. |
| Keywords: | Rat Brain areas Serotonin Electrochemical detection High pressure liquid chromatography Method |
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