河南汉族人群11个STR基因座在排除父权方面的应用

目的 :分析 11个短串联重复序列 (shorttandemrepeats,STR)D12S391、vWA、D5S818、D16S5 39、D7S82 0、F13A0 1、D13S317、FESFPS、CSF1PO、TPOX、THO1在河南省汉族群体亲子鉴定事件中排除父权方面的应用价值。方法 :对 30 4例做亲子鉴定的河南汉族个体的DNA样本应用多位点复合PCR扩增技术及聚丙烯酰胺凝胶电泳分型方法 ,结合PowerStatssoftware软件 ,分析上述 11个STR的观察杂合度 (Ho)、个体识别率 (DP)、多态性信息含量 (PIC) ,并计算其参与联合排除父权的比率。结果 :11个STR参与 36例联合排除父权的比率依次为 6 9.4 % ,5 2 .8% ,4 7.2 % ,4 7.2 % ,4 4 .4 % ,33.3% ,33.3% ,33.3% ,2 7.8% ,2 2 .2 % ,16 .7% ,其中 6个 (D12S391、D7S82 0、vWA、D5S818D13S317、D16S5 39)Ho>0 .7、DP >0 .9、PIC >0 .7。结论 :短串联重复序列D12S391、D7S82 0、D5S818、vWA、D16S5 39可作为亲子鉴定的首选基因座 ,在法医学应用和群体遗传学研究中有较高的价值

Application of eleven STR loci for excluding paternity in Henan Han population

Aim: To analyze the application value of short tandem repeats(STR D12S391, D7S820, vWA, D5S818, F13A01, D13S317, D16S539, CSF1PO, FESFPS, TPOX, THO1) for excluding paternity in forensic test in Henan Han population. Methods: DNA samples of 760 Henan Han person from 304 forensic test cases were extracted, then 11 STR were amplified and electrophoresed on denaturation polyacrylamid gel for allele genotypes. The observed heterozygosities (Ho), power of discrimination (DP), polymorphism information content (PIC) were calculated by Power Stats software for each locus, and the participating frequency of STR, which participated the case of excluding paternity cooperatively were also calculated. Results: The participating frequency of 11 STR which participated the case of excluding paternity cooperatively were as following: 69.4%,52.8%,47.2%,47.2%,44.4%,33.3%,33.3%,33.3%,27.8%,22.2%,16.7%.Among 11 STR, there were 6 loci(D12S391, D7S820, vWA, D5S818, D13S317, D16S539)with Ho>0.7, DP>0.9, PIC>0.7. Conclusion: The participating frequency of D12S391, vWA, D7S820, D5S818, D16S539 are higher, and their Ho, DP, and PIC are higher too. So, these loci are the first chosen ones in paternity test in Henan Han population.