流体剪切力通过Piezo1调控MG-63骨肉瘤细胞的凋亡

目的:探讨流体剪切力（FSS）对Piezo1的影响以及调控MG-63细胞凋亡的机制。方法:首先分两组Control和FSS组，FSS组施加FSS，Control组不干预，使用Western Blot检测两组caspase3和Piezo1的表达。再将细胞分为Control组、FSS组、GsMTx4组和FSS+GsMTx4组，FSS组加载FSS，GsMTx4组给予GsMTx4处理，FSS+GsMTx4组先给予GsMTx4处理后再加载FSS，检测各组凋亡相关蛋白caspase3、caspase9、BAD和Bax的表达水平。结果:60 min 12 dyn/cm2的FSS可上调MG-63细胞caspase3和Piezo1的表达，此外，FSS可通过上调Piezo1的水平从而促进caspase3、caspase9、BAD和Bax的表达水平。结论:FSS可通过Piezo1机械敏感离子通道促进MG-63人骨肉瘤细胞的凋亡，Piezo1可能是一种治疗骨肉瘤的新型分子靶点。

Fluid shear stress regulates apoptosis of MG-63 osteosarcoma cells through Piezo1

Abstract: Objective To investigate the effect of fluid shear stress (FSS) on Piezo1 and the mechanism of FSS regulating the apoptosis of MG-63 cells. Methods Cells were firstly divided into two groups, namely control group and FSS group. The expressions of caspase3 and Piezo1 were detected by Western Blot after interventions were applied to each group. Then the cells were divided into control group, FSS group, GsMTx4 group and FSS+GsMTx4 group. In FSS+GsMTx4 group, cells were intervened with GsMTx4 first and FSS second. The expression levels of caspase3, caspase9, BAD and Bax were detected after different interventions. Results FSS at 12 dyn/cm2 for 60 min could up-regulate the expression of caspase3 and Piezo1 in MG-63 cells. Besides, FSS could promote the expression levels of caspase3, caspase9, BAD and Bax by up-regulating the expression of Piezo1. Conclusion FSS can promote the apoptosis of MG-63 human osteosarcoma cells through Piezo1 mechanosensitive ion channels. Piezo1 may be a new molecular target for the treatment of osteosarcoma.