甲氟喹对胶质瘤细胞的放射增敏作用

目的:研究甲氟喹对胶质瘤细胞的放射增敏作用，并对其机制进行初步探索。方法:取指数生长期的胶质瘤U251细胞，采用CCK-8检测法研究甲氟喹作用后U251细胞的存活率；细胞克隆形成实验评估甲氟喹对U251细胞的放射增敏效果；流式细胞技术检测甲氟喹联合X射线作用后U251细胞的活性氧水平和细胞凋亡率，探索联合作用下U251细胞的死亡机制。结果:甲氟喹对胶质瘤细胞具有良好的放射增敏效果，其对U251细胞的最大放射增敏比为1.64。X射线联合甲氟喹作用于U251细胞后，细胞的凋亡水平增加，活性氧水平增加，活性氧抑制剂谷胱甘肽能抑制X射线联合甲氟喹作用诱导的U251细胞凋亡。结论:甲氟喹对U251细胞有放射增敏作用，其作用机制可能为通过增加细胞活性氧水平、诱导细胞凋亡导致放射敏感性增加。

The radiosensitization effect of mefloquine combined with X-ray on glioma cells

Objective To study the radiosensitization effect of mefloquine on glioma cells and to explore its mechanism.Methods The glioma U251 cells at the exponential growth period were sampled and their survival rate after mefloquine action was studied by CCK-8 assay;cell clone formation experiments were used to evaluate the radiosensitization effect of mefloquine on U251 cells;flow cytometry was used to detect the active oxygen level and apoptosis rate of U251 cells after mefloquine combined with X-ray,and the death mechanism of U251 cells under the combined effect was explored.Results Mefloquine had a good radiosensitization effect on glioma cells,and its maximum radiosensitization ratio to U251 cells was 1.64.After X-ray combined with mefloquine acted on U251 cells,the level of cell apoptosis increased,and the level of reactive oxygen species increased.The active oxygen inhibitor glutathione can inhibit U251 cell apoptosis induced by X-ray combined with mefloquine.Conclusion Mefloquine has a radiosensitizing effect on U251 cells,and it is probably through increasing the level of cellular reactive oxygen species and inducing apoptosisthat mefloquinecan lead to increased radiosensitivity.