广东省凤尾蕨属植物抗白血病药物研究

目的 探讨广东地区5种常见凤尾蕨属植物半边旗、剑叶凤尾蕨、刺齿凤尾蕨、蜈蚣草和井栏边草对白血病细胞生长的抑制作用.方法 用乙醇经索氏提取器分别提取5种植物的有效成分,以二甲基亚砜分别配制供试品溶液;将供试品溶液加入对数生长期的人早幼粒白血病HL-60细胞株(简称HL-60细胞)和人慢性髓样白血病K562细胞株(简称K5...

Anti-Leukemia Effect of Pteris Plants from Guangdong Province

Objective To investigate the inhibitory effects of five common Pteris plants including Pteris semipinnata,Pteris e nsiformis,Pteris dispar,Pteris vittata and Pteris multifida from Guangdong Province on the growth of leukemia cell lines.Methods The active ingredients from five Pteris plants were extracted with ethanol by the Soxhlet’s extractor,and the test solution was prepared with dimethyl sulfoxide(DMSO).The test solution was added to the human promyelocytic leukemia HL-60 cell line(hereinafter referred to as HL-60 cells) and human chronic myeloid leukemia K562 cell line(hereinafter referred to as K562 cells) during the logarithmic growth stage,and the cell survival rate was counted by the trypan blue staining.Results Pteris semipinnata,Pteris ensiformis,Pteris dispar,Pteris vittata and Pteris multifida could inhibit the growth of HL-60 cells and K562 cells.Among them,Pteris semipinnata and Pteris dispar showed the stronger inhibition on the growth of HL-60 cells,and the survival rate of HL-60 cells was zero in 4.0 g/L of ethanol extracts of Pteris semipinnata and Pteris dispar.Pteris semipinnata and Pteris vittata showed the stronger inhibition on the growth of K562 cells,and the survival rate of K562 cells was zero in 4.0 g/L of ethanol extracts of Pteris semipinnata and Pteris vittata.Conclusion The inhibitory effect of five Pteris plants on the growth of HL-60 cells is stronger than that on the growth of K562 cells.In addition to the Pteris semipinnata,Pteris dispar and Pteris vittata may be potential anti-leukemia herbs.