GC/MS法同时检测生物样品中苯丙胺、甲基苯丙胺及MDMA

目的:建立体液和组织中苯丙胺、甲基苯丙胺和3,4 -亚甲基二氧基甲基苯丙胺(MDMA)的简便快速、灵敏可靠的GC/MS检测方法。方法:样品经碱化,乙醚提取,TFA衍生化后,GC/MS全扫描定性检测,选择离子扫描(SIM)定量分析。结果:几种药物在血浆中的线性范围为0 .0 2 - 2 . 0 μg·ml-1,最低检出浓度为0 . 0 0 5 μg·ml-1(S/N≥3) ;肝脏中线性范围为0 . 0 5 - 5. 0 μg·g-1,最低检出浓度0. 0 .10 μg·g-1(S/N≥3)。相关系数大于或等于0 9980 ;回收率在92. 1% - 10 5. 2 %之间。结论:GC/MS方法适用于生物样品中苯丙胺、甲基苯丙胺和MDMA浓度的检测。

SIMULTANOUS DETERMINATION OF AMPHETAMINE,METHAMPHETAMINE AND MDMA IN BIOLOGICAL SPECIMENS BY GC/MS

Objective: To improve a GC/MS method for simultaneous determination of amphetamine, methamphetamine and 3, 4-methylenedioxymethamphetamine(MDMA) in body fluid and tissue. Methods: The specimens were alkalinized and extracted with ether, the extracts were derivatized with TFA and full scan for qualitative analysis and selection scan for quantitative analysis by GC/MS. Results: The calibration of amphetamine, methamphetamine and MDMA had a good linear relationship from 0.02 μg·ml -1 to 2.0 μg·ml -1 and the minimal detection concentration was 0.005 μg·ml -1 in the plasma. The liner ranges of these drugs were 0.05-5.0 μg·g -1 and the minimal detection concentration was 0.010 μg·g -1 in the liver. All the coefficients were not less than 0.9980 and the recoveries were 92.1 %-105.2 %. Conclusion: This method can be used to detect amphetamine, methamphetamine and MDMA concentrations in biological specimens.