院内外环境分离金黄色葡萄球菌的耐药性及毒素基因检测

目的检测医院及环境样中金黄色葡萄球菌（Staphylococcus aureus,SA）耐药基因及毒素基因,了解其分布情况。方法采用多重聚合酶链反应（multiplex polymeras chain reaction,PCR）技术分别检测耐药基因mecA、femA与毒素基因TSST、PVL。结果 83株金黄色葡萄球菌,仅3株（3.61%）菌检出mecA基因,22株菌（26.50%）检出femA基因,15株菌（18.07%）同时检出mecA与femA基因。医院样本检出12株耐甲氧西林金黄色葡萄球菌（methicillin-resistantStaphylococcus aureus,MRSA）,3株甲氧西林耐药凝固酶阴性的金黄色葡萄球菌（methicillin-resistant and coagulasenegative Staphylococcus aureus,MRCNS）。83株分离自医院及环境样本SA,2株分离自咽拭子的样本检出TSST基因,检出率4.54%（2/44）;1株分离自血液的样本检出PVL基因,检出率2.27%（1/44）,其余菌株未检出毒素基因。结论院内样本中,部分菌株携带mecA基因,环境样本未发现携带mecA菌株,两者存在差异。83株SA菌,毒素基因携带率较低。检测耐药、毒素基因携带率,为临床治疗及院内感染控制,食物中毒溯源提供依据。

Detection of antibiotic genes and toxic genes of Staphylococcus aureus

Objective To detect the antibiotic and toxic genes of Staphylococcus aureus in hospital and environment.Methods The antibiotic genes mecA,femA and toxic genes TSST,PVL of Staphylococcus aureus were detected separately by multiplex polymerase chain reaction（PCR）.Results In all 83 Staphylococcus aureus strains,only 3 strains（3.61%） carried mecA genes,22 strains（26.50%） carried femA genes,15 strains（18.07%） carried mecA and femA genes.12 MRSA strains and 3 MSCNS strains were detected from samples from hospital,44 strains Staphylococcus aureus were isolated from samples of hospital and environment,2 strains from samples of Throat swabs carried TSST gene（4.54%,2/44） and 1 strains from sample of blood carried PVL gene（2.27%,1/44）,all of the rest strains did not carry the two toxic genes.Conclusion Some strains from hospital samples carry mecA genes,but those isolated from environment do not carry this gene.The detection rate of toxic genes were lower in 83 Staphylococcus aureus strains.