猴艾滋病模型CD28家族mRNA动态变化及中药干预作用

目的观察猴艾滋病毒(SIV)感染猴模型外周血CD28家族免疫共刺激分子mRNA动态变化及中药复方艾可清的干预作用。方法 SIVmac251感染12只恒河猴,分别于感染前、感染后8周每周、给药前(SIV感染第10周)、给药后每4周时间点采集外周静脉血,分离PBMC,以定量PCR检测CD28、ICOS、PD-1、CTLA-4及HLA-DRmRNA。结果所观察的4种免疫共刺激分子及免疫活化标志物HLA-DR mRNA量出现了不同程的升高,其中HLA-DRmRNA高峰出现在SIV感染后第2周,而4种免疫共刺激分子mRNA峰值均出现在SIV感染第5周之后。艾可清使ICOS、CD28、PD-1及HLA-DRmRNA量出现不同程度的降低,而对CTLA-4mRNA量无显著影响。结论 SIV感染猴外周血PBMC负性和正性CD28家族免疫共刺激分子mRNA均出现不同程度的升高;艾可清可能通过同时降低正性及负性免疫共刺激分子mRNA表达而减轻艾滋病免疫活化。

Dynamic changes of CD28 family immune co-stimulatory molecular mRNA expression in SIV infected monkeys and intervention effects of Chinese Medicine

Objectives To observe the CD28 family immune co-stimulatory molecular mRNA expression dynamic changes in PBMC of SIV infected monkeys and evaluation of the intervention effects of Chinese Medicine.Methods 12 rhesus monkeys were infected with SIVmac251.Peripheral vein blood were collected at the time points of 1 day before SIV infection,each week in 8 weeks post SIV infection,1 day before drug administration,one time in 4 weeks post drug administration and drug cessation.PBMC were isolated and mRNA of CD28,ICOS,PD-1,CTLA-4 and HLA-DR were tested with quantitative PCR methods.Results All of the 4 CD28 family co-stimulatory molecular and HLA-DR mRNA were elevated in different extent.The summit values of all the 4 CD28 family co-stimulatory molecular mRNA occurred at the time points of 5 weeks post SIV infection,while the summit value of HLA-DR mRNA occurred 2 weeks post SIV infection.The mRNA values of ICOS,CD28,PD-1 and HLA-DR are significantly down-regulated after AiKeQing administration.But the CTLA-4 mRNA didn＇t show marked change.Conclusion Both the positive and negative modulation CD28 family co-stimulatory molecular mRNA are significantly up-regulated after SIV infection.The AIDS immune activation may be relieved by AiKeQing through down-regulation of both the positive and negative modulation CD28 family co-stimulatory molecular.