深圳市2010年HIV-1分子流行病学调查

目的了解2010年艾滋病病毒-1型（HIV-1）毒株亚型在深圳市的流行情况,为预防和控制HIV在本市的流行提供有价值的资料。方法收集2010年深圳市HIV-1确认为阳性的样本150例,应用巢式聚合酶链反应（nested-PCR）技术,对该样本膜蛋白基因（env基因）和核心蛋白（gag基因）进行扩增,并对各基因区核苷酸序列进行测定和分析。结果共有128份样本获得分型结果,其中CRF01_AE重组株占63.3%（81/128）,B’亚型占14.1%（18/128）,CRF07_BC重组株占13.3%（17/128）,CRF08_BC重组株占7.0%（9/128）,C亚型占0.8%（1/128）,A1亚型占1.6%（2/128）;CRF01_AE重组株以吸毒传播、异性性传播和同性性传播为主,分别占其感染人群的23.5%、45.7%和30.9%,CRF07_BC、CRF08_BC重组株以异性性传播和同性性传播为主,分别占各感染人群的41.2%、58.8%和66.7%、33.3%%,B’亚型主要分布在性传播、献/输血和母婴传播人群。本文首次在吸毒人群中发现A1亚型。结论 2010年深圳市的HIV-1是以CRF01_AE重组株、B’亚型、CRF07_BC和CRF08_BC重组株为主要流行毒株,也存在A1和C亚型;CRF01_AE重组株是各传播途径人群中的主要流行株,并且CRF01_AE重组株在各人群中所占的比例高于国内其他地区。

Molecular epidemiology of HIV-1 subtypes in Shenzhen of 2010

Objective To study the prevalence status of human immunodeficiency virus-1（HIV-1）subtypes in Shenzhen and study their source in order to predict the epidemic trend.Methods Samples were collected from 150 newly reported HIV-1 infected in Shenzhen in 2010.HIV-1 env gene and gag gene were amplified by nested-PCR from RNA.The C2-V3 region of HIV-1 env and P24 region were sequenced for analyses.Results Among 128 samples,there were 6 HIV-1 strains including CRF01_AE,CRF08-BC,CRF07-BC 3 circulating recombinant forms（CRFs）and B＇,C,A1 3 subtypes.Subtyping showed that 63.3%（81/128）were CRF01-AE,14.1%（18/128）were subtype B＇,13.3%（17/128）were CRF07_BC,7.0%（10/122）were CRF08_BC,0.8%（1/128）were subtype C,1.6%（2/128）were subtype A1.And CRF01_AE strain mainly from injecting drug users（IDUs）and heterosexuals and homosexuals.CRF07_BC and CRF08_BC strains mainly from heterosexual and homosexual.Subtype B＇ were mainly from heterosexuals and homosexuals,blood donors and mothers to children.And subtype A1 was first found in IDUs.Conclusion CRF01_AE、subtype B＇,CRF07_BC and CRF08_BC of HIV-1 strains were mainly epidemic subtypes in Shenzhen in 2010.Subtype C and A1 were also circulating in Shenzhen region.CRF01_AE recombinant was largely predominant among all transmitting population.And the proportion of CRF01_AE in various groups of population is higher than that in other regions of China.