一期梅毒半巢式聚合酶链反应检测的效果

目的 探讨半巢式聚合酶链反应（PcR）在一期梅毒早期诊断中的意义。方法应用半巢式PCR检测一期梅毒溃疡组织液和血清中TP-DNA，与IPPA结果进行比较。结果60例临床诊断为一期梅毒患者，溃疡组织液、血清半巢式PCR和TPPA检测的阳性分别为56例（93．33％）、13例（21．67％）和53例（88．33％）。组织液半巢式PCR检测结果与血清半巢式PCR检测结果比较，差异有统计学意义（P〈0．05），与rI胛A结果比较，差异无统计学意义（D0．05）。结论半巢式PCR检测一期梅毒溃疡组织液中TP-DNA较检测血清中的TP-DNA敏感性高、特异强，可做为TPPA诊断早期梅毒的有效补充。

Application of semi-nested PCR for diagnosis of primary syphilis

Objective To explore semi-nested polymerase chain reaction （semi-nested PCR）in diagnosis of primary syphilis. Methods Ulcer secretion and blood sample were collected from primary syphilis patients, and semi-nested PCR was used to identify the presence of TP-DNA. The positive rates were compared with treponema pallidum particle assay （TPPA）. Results Samples were collected from 60 patients. The positive rates of semi-nested PCR for ulcer secretion, serum and TPPA were 93.33% ,21.67% and 88.33% , respectively.There was statistically significance （P〈0.OS）between semi-nested PCR determiantion of ulcer secretion and semi-nested PCR determination of serum, but there was no significant differences in the comparison with TPPA （P〉0.05）. Conclusion The sensitivity and specificity of semi-nested PCR for determiantion of ulcer secretion were higher than that of semi-nested PCR for determination of serum, therefore it can be a supplement for diagnosis of early syphilis by TPPA.