人芽囊原虫感染小鼠血清中IL-17水平的动态观察 |
| |
引用本文: | 符瑞佳,伍玲园,卢作超,唐莉莉,刘腾,张璠,刘登宇. 人芽囊原虫感染小鼠血清中IL-17水平的动态观察[J]. 中国热带医学, 2012, 12(1): 24-27 |
| |
作者姓名: | 符瑞佳 伍玲园 卢作超 唐莉莉 刘腾 张璠 刘登宇 |
| |
作者单位: | 1. 广西医科大学寄生虫学教研室,广西南宁,530021 2. 广西医科大学基础医学院,广西南宁,530021 |
| |
摘 要: | 目的 观察小鼠感染人芽囊原虫后血清中白细胞介素17(IL-17)水平的动态变化,从细胞因子的角度来探讨IL-17在人芽囊原虫感染中的意义。方法BABL/C小鼠120只随机分为实验组、免疫抑制剂组、对照组,每组各40只,三组小鼠分别于感染后第2d、3d、4d、5d、6d、7d、8d、10d、12d、14d各取4只,采用ELISA试剂盒检测小鼠血清中IL-17水平。结果免疫抑制剂组和对照组小鼠血清中IL-17的含量在不同感染时期基本处于稳定水平(P〉0.05),而感染后不同时期的实验组小鼠血清IL-17水平出现升高,差异有统计学意义(P〈0.05),其中第4d、5d、6d的表达分别高于免疫抑制剂组和对照组(P〈0.05),且第5d达到高峰。结论提示了IL-17可能在人芽囊原虫早期感染中发挥了重要作用。
|
关 键 词: | 人芽囊原虫 白细胞介素17 血清 动态变化 |
Variation of level of serum IL-17 in mice infected with Blastocystis hominis |
| |
Affiliation: | Variation of level of serum IL-17 in mice infected with Blastocystis hominis. FU Rui-jia,WU Ling-yuan,LU Zuo-chao,et al. (Department of Parasitology,Guangxi Medical University,Nanning 530021,Guangxi,P. R. China; Correponding author:LIU De ng-yu, E-mail: 33547533 @qq.corn ) |
| |
Abstract: | Objective To observe the dynamic changes of IL-17 in mice infected with Blastocystis hominis. Methods 120BABIEC mice were randomly divided into experimental groups, immunosuppressant group and normal group. Four BABL/C mice from each group were randomly sacrificed on the day 2,3,4,5,6,7,8,10,12 and 14 after infection,respectively. ELISA was used to measure the serum levels of IL-17 of these mice. Results The level of serum IL-17 of immunosuppressant group and normal group maintained relatively stable throughout the experiment (/:'〉0.05). The level of serum IL-17 of experiment group began to rise apparently after infection (P〈0.05) at different stages of experiment. The level of serum IL-17 of experiment group showed a great increase on day 4, day 5 and day 6(P〈0.05),and the IL-17 concentration in the serum of mice rose to a peak on the day 5. Conclusion The level of serum IL-17 in mice increased afer being infected with Blastocystis hominis ,and reached a peak on the day 5, then decreased. IL-17 may play an important role in the early infection of mice with Blastocystis hominis. |
| |
Keywords: | Blastocystis hominis Interleukin-17 Serum Dynamic change |
本文献已被 维普 万方数据 等数据库收录! |
|