广州市首例利齐菲尔德沙门菌食物中毒的病原学分析

目的对2011年7月广州市发生的一起细菌性食物中毒进行病原学特征分析。方法对送检病人粪便、厨工肛拭及食物等样品进行荧光PCR检测和分离培养,对分离株进行生化鉴定,血清分型、脉冲场凝胶电泳（PFGE）分型和药敏分析。结果从病人粪便、肛拭和厨房抹布共分离到18株利齐菲尔德沙门菌,为相同的PFGE型别,药敏结果显示菌株对实检和定阿米卡星,庆大霉素及妥布霉素耐药,对头孢噻肟、亚胺培南、甲氧苄啶/磺胺甲恶唑等15种抗生素均敏感。结论实验判定这是一起由同一克隆型的利齐菲尔德沙门菌污染厨房环境所引起的食物中毒事件,PFGE分型法为暴发溯源提供分子流行病学证据和支持。

Analysis of the pathogenic characteristics of the first food-poisoning outbreak caused by Salmonella Litchfield in Guangzhou

Objective To study the pathogenic characteristics of Salmonella enterica serovar Litchfield isolated from a food-poisoning outbreak.Methods Real-time PCR,Biochemistry identification,serotyping,pulse-field gel electrophoresis（PFGE）and drug sensitivity test were applied to detect the pathogen and to trace the possible strains.Results Eighteen Salmonella Litchfield strains were isolated from the patients＇ fecal,stool and a piece of kitchen cloth,which were classified as the same PFGE type.All strains were resistant to Amikacin,Gentamicin and Toramycin,but sensitive to Cefotaxime,Imipenem,Trimethoprim/sulfamethoxazole,et al.Conclusions One Salmonella Litchfield clone was responsible to the food poisoning outbreak.PFGE gives molecular epidemiological evidence and support for the source tracing of an outbreak.