| 家蝇抗菌肽Cecropin对人源性肿瘤细胞增殖与凋亡的影响 |
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| 引用本文： | 金小宝,龚水明,蒲俏虹,朱家勇,褚夫江,梅寒芳.家蝇抗菌肽Cecropin对人源性肿瘤细胞增殖与凋亡的影响[J].中国热带医学,2012,12(3):293-295. |
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| 作者姓名： | 金小宝 龚水明 蒲俏虹 朱家勇 褚夫江 梅寒芳 |
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| 作者单位： | 1. 广东药学院基础学院,广东广州510006;广东省生物活性药物研究重点实验室,广东广州510006
2. 广东药学院附属门诊部,广东广州,510224 |
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| 基金项目： | 广东省社会发展项目，广州市社会发展攻关项目 |
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| 摘 要： | 目的分析家蝇抗菌肽Cecropin对人源性肿瘤细胞体外生长增殖与凋亡的影响。方法采用四甲基偶氮噻唑蓝（MTT）MTT比色法测定家蝇抗菌肽Cecropin对人肺癌细胞株A549、人乳腺癌细胞株MCF-7、人宫颈癌细胞株Hela、人肝癌细胞株BEL-7402和人正常肝细胞株Changs,Liver生长增殖情况的影响,采用流式细胞术检测家蝇抗菌肽Cecropin作用后4株肿瘤细胞凋亡的情况,对照组不加家蝇抗菌肽Cecropin。结果家蝇抗菌肽Cecropin对4株人源性肿瘤细胞的生长均有抑制作用,并能诱导肿瘤细胞发生凋亡,但对人肝癌细胞BEL-7402作用效果相对较强。结论家蝇抗菌肽Cecropin能够影响人源性肿瘤细胞的生长与凋亡,作用机制需进一步研究。
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| 关 键 词： | 家蝇 抗菌肽 肿瘤细胞 增殖 凋亡 |
Effect of antimicrobial peptide cecropin of Musca domestica on proliferation and apoptosis of human tumor cell |
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| Institution: | JIN Xiao-bao,LI Xiao-bo,ZHU Jia-yong,,et al.(1 School of Basic Acinecs,Guangdong Pharmaceutical University,Guangzhou 510006,Guangdong,P.R.China) |
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| Abstract: | Objective To observe the effect of antimicrobial peptide cecropin of Musca domestica on proliferation and apoptosis of human tumor cells in vitro.Methods The effect of housefly antimicrobial peptide cecropin on human lung cancer cell line A549,human breast cancer cell line MCF-7,human cervical cancer cell line Hela,human hepatoma cell line BEL-7402 and human normal liver cell line Changs,Liver was investigated by MTT colorimetric assay.The apoptosis of tumor cell were investigated by flow cytometry,and control group without cecropin.Result The growth of human tumor cells was inhibited by cecropin,and cecropin could induce apoptosis of tumor cells,but the effect on human liver cancer BEL-7402 cells was the strongest.Conclusion Housefly antimicrobial peptide cecropin can affect growth and apoptosis of human tumor cell,but the mechanism needs further studying. |
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| Keywords: | Musca domestica Antimicrobial peptide Tumor cell Proliferation Apoptosis |
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