| Ghrelin在重症急性胰腺炎并发肾损伤中的作用 |
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| 引用本文： | 杨慧莹,苏娟,唐国都,覃蒙斌,梁志海,钟卫一. Ghrelin在重症急性胰腺炎并发肾损伤中的作用[J]. 广西医学, 2010, 32(7): 759-762 |
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| 作者姓名： | 杨慧莹 苏娟 唐国都 覃蒙斌 梁志海 钟卫一 |
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| 作者单位： | 广西医科大学第一附属医院消化内科,南宁市,530021 |
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| 摘 要： | 目的探讨Ghrelin在重症急性胰腺炎（SAP）并肾损伤中的作用以及外源性褪黑素对其的影响。方法 72只大鼠随机分为对照组（C组,n=24）、SAP组（A组,n=24）和褪黑素干预组（M组,n=24）。A组分3次腹腔内注射6%L-精氨酸（L-Arg）1.5g·kg^-1·次^-1建立SAP模型;C组同法注射等量生理盐水;M组于首次注射L-Arg前0.5h腹腔内注射1%褪黑素50μg/kg。各实验组大鼠于末次腹腔内注射后6h、12h和24h分批处死。光镜下对胰腺和肾组织进行病理学评分,用血清淀粉酶试剂盒测定血清淀粉酶,用ELISA检测血清IL-6,应用RT-PCR检测肾脏Ghrelin及NF-κB p65mRNA表达。结果 A组各时点胰腺及肾脏病理评分、血清淀粉酶及IL-6、肾脏NF-κB p65mRNA表达均较C组显著升高（P〈0.05）,而同时点Ghrelin mRNA表达较C组明显降低（P〈0.05）;M组各时点胰腺及肾脏病理评分、血清淀粉酶及IL-6、肾脏NF-κB p65mRNA表达均较A组显著降低（P〈0.05）,而同时点Ghrelin mRNA表达较A组升高（P〈0.05）。结论 Ghrelin在急性胰腺炎并肾损伤时的表达可能与病变严重程度有关。外源性褪黑素可能通过抑制NF-κB p65的表达而减轻急性胰腺炎并肾损伤,并可能使Ghrelin在肾脏中表达增多。
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| 关 键 词： | 胰腺炎 肾损伤 Ghrelin 核因子-κB p65 褪黑素 |
Roles of Ghrelin in Severe Acute Pancreatitis Associated with Kidney Injury |
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| YANG Hui-ying,SU Juan,TANG Guo-du,QIN Meng-bin,LIANG Zhi-hai,ZHONG Wei-yi. Roles of Ghrelin in Severe Acute Pancreatitis Associated with Kidney Injury[J]. Guangxi Medical Journal, 2010, 32(7): 759-762 |
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| Authors: | YANG Hui-ying SU Juan TANG Guo-du QIN Meng-bin LIANG Zhi-hai ZHONG Wei-yi |
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| Affiliation: | YANG Hui-ying,SU Juan,TANG Guo-du,QIN Meng-bin,LIANG Zhi-hai,ZHONG Wei-yi(Department of Gastroenterology,First Affiliated Hospital,Guangxi Medical University,Nanning 530021,China) |
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| Abstract: | Objective To investigate the roles of Ghrelin in acute necrotizing pancreatitis(SAP)associated with kidney injury in rats,and assess the effect of ectogenic melatonin on Ghrelin activity.Methods Seventy-two SD rats were randomly divided into control group(group C,n=24),SAP group(group A,n=24)and melatonin intervention group(group M,n=24).In group A,the rats were injected intraperitoneally three times with 6%L-arginine(1.5 g/kg) in an interval of 1 hour for SAP.In group C,the rats received the same amount of... |
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| Keywords: | Ghrelin |
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