表皮生长因子对大鼠移植小肠形态及功能的作用研究

目的：了解表皮生长因子（epidermal growth factor，EGF）对大鼠移植小肠萎缩及功能低下的预防作用。方法：近交系Wistar(RT1^k)大鼠行异位全小肠移植后自第2天开始给予肠外营养至第10天，对照组（n=10）行常规TPN(total parenteral nutrition)支持，实验组（n=10）行常规TPN支持的同时加用表皮生长因子。结果：实验组移植肠绒毛高度、隐窝深度、粘膜厚度及绒毛表面积均明显大于对照组，肠上皮细胞超微结构基因保持完好，对照组则出现明显线粒体肿胀和微绒毛萎缩。实验组血浆^15N-甘氨酸丰度明显于对照组。结论：表皮生长因子能维护大鼠移植小肠粘膜形态与功能，改善肠管对氨基酸的吸收。

The effect of epidermal growth factor on graft small bowel morphology and absorption in rats

Objective To study the effect of epidermal growth factor(EGF) on the morphology and absorption of small bowel graft. Method Total small bowel transplantion was performed between inbred Wistar(RT1 k) rats. Total parenteral nutrition(TPN group,n=10) and EGF supplemented TPN(EGF group,n=10) was used from POD2 to POD10. ResultsMucosal villous height,crypt depth, mucosal thickness and villous surface area were found significantly larger in EGF group than in TPN group.Normal ultrastructure of the graft was maintained in EGF group, Atrophied microvilli and broken mitochondrial crista were observed in TPN group. Plasma 15N-Glycine level was higher in EGF group than in TPN group. Conclusion EGF improves mucosal morphology,preserves enterocyte ultrastructure, enhances amino acid absorption.