表皮生长因子受体和Ki67及p16在成人中耳胆脂瘤中的表达

目的:研究表皮生长因子受体(EGFR)、Ki67、p16在成人中耳继发性胆脂瘤上皮中的表达情况,分析它们之间的相互关系,探讨其表达对胆脂瘤上皮侵袭能力的影响。方法:应用免疫组织化学SP染色方法检测EGFR、Ki67和p16在30例成人中耳胆脂瘤上皮、21例成人胆脂瘤患者外耳道正常皮肤、17例正常人外耳道中的表达情况,应用计算机图像分析系统对其阳性表达进行定量分析。结果:EGFR、Ki67、p16在成人中耳继发性胆脂瘤上皮中阳性表达率分别为70.0%,60.0%,46.7%,与外耳道正常皮肤相比表达均差异有统计学意义。成人中耳胆脂瘤中EGFR、Ki67与p16之间表达均无相关性(均P>0.05)。胆脂瘤侵袭能力与EGFR、Ki67表达有显著相关性(均P<0.01)。EGFR、Ki67表达灰度值越低,表达密度越高,胆脂瘤侵袭能力越强。p16在成人中耳胆脂瘤中的表达与侵袭能力之间无相关性(P>0.05)。EGFR、Ki67、p16在成人中耳胆脂瘤中阳性细胞主要分布于上皮全层,以基底层和棘层为著,呈高度表达;而在对照组中阳性细胞仅在基底层表达,呈弱表达。结论:EGFR、Ki67、p16在成人中耳胆脂瘤中呈高表达,EGFR、Ki67的表达与成人中耳胆脂瘤的侵袭能力有高度相关性,提示成人中耳胆脂瘤具有高度增殖能力,其中细胞因子EGFR、Ki67、p16起到重要的作用。

Expression of epidermal growth factor receptor Ki67 and p16 in human middle ear cholesteatoma

Objective:This study aimed to analyze the expression of epidermal growth factor receptor (EGFR), Ki67 and p16 in human middle ear cholesteatomas and to investigate the correlation between its expression and the ability of erosion of cholesteatoma. Method:The specimens from the acquired middle ear cholesteatoma tissue of 30 cases and 21 external ear canal skin samples from patients and 17 external ear canal skin samples from healthful men were taken intraoperatively. Their expression was examined by immunohistochemical SP method. Then we scanned it into a computer by an image scanner and quantified the gray of them using commercial software. Result:The percent of positive expression of EGFR, Ki67 and p16 in middle ear cholesteatoma were 70.0%,60.0%,46.7%.Their expression tended to the increased greatly compared with the skins of the control groups. There was not correlation between the expression of EGFR,Ki67 and p16 (P>0.05).It showed statistically significant correlation between expression of EGFR and the ability of erosion of middle ear cholesteatoma (P<0.01).There was correlation between the expression of Ki67 and the ability of erosion of middle ear cholesteatoma too (P<0.01). But there was not correlation between the expression of p16 and the ability of erosion of middle ear cholesteatoma (P>0.05).The expression of EGFR ,Ki67,p16 in all epithelial layers of middle ear cholesteatoma were abundantly stained, especially in the basal and spinous layers. Only the basal layer were slightly stained in control groups. Conclusion:The expression of EGFR,Ki67,p16 in middle ear cholesteatoma was significantly higher compared with the skin of external auditory of cholesteatoma patients and healthful peoples. There was correlation between the expression of EGFR or Ki67 and the ability of erosion of middle ear cholesteatoma. It means that EGFR,Ki67and p16 play a key role in the hyperproliferation middle ear cholesteatoma.