石斛合剂调控老年糖尿病大鼠GLP-1的研究

目的] 观察石斛合剂(DC)对老年糖尿病大鼠胰高血糖素样多肽-1(GLP-1)的影响,探讨石斛合剂治疗衰老糖尿病大鼠的作用机制。方法] 老年Wistar雌性大鼠分为正常对照组、模型组、DC低剂量组、DC高剂量组、二甲双胍对照组,检测0.5、2.5 h血清GLP-1,免疫组化法检测小肠GLP-1.结果] DC低剂量组0.5、2.5 h GLP-1的差值低于模型组(P<0.05),DC高剂量组差值显着高于模型组(P <0.01),小肠未发现GLP-1.结论] 低剂量DC可能具有减缓GLP-1代谢的作用,高剂量DC可能具有促进0.5 h GLP-1分泌的作用。

Studies on dendrobium compound control GLP-1 in aged diabetes mellitus rat

Objective] Observed the influence of Dendrobium compound(DC) affected on GLP-1 expression on aged diabetes. Methods] Female Wistar rats were divided into normal control group, model group, DC Mixture low dose group, DC Mixture high dose group, metformin control group. Detect 0.5 and 2.5 h GLP-1 of each group. Immunohistochemistry was used to evaluate the protein expression of GLP-1.Results] The difference between 0.5 and 2.5 h of GLP-1 in the DC low group were lower than the molding group(P<0.05); the difference between 0.5 and 2.5 h of GLP-1 in the DC high group were higher markedly than the molding group(P <0.01);GLP-1 was not found in the small intestine.Conclusion] The DC low group may slow down the metabolism of GLP-1, and the DC high group may promote the secretion of GLP-1 in 0.5 h.