Epitope-specific impairment of production of antibody against merozoite surface glycoprotein 1 of Plasmodium falciparum in symptomatic patients with malaria |
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| Authors: | Jun Fu Mariko Hato Hiroshi Ohmae Hiroyuki Matsuoka Masato Kawabata Kazuyuki Tanabe Yoshitaka Miyamoto Judson L. Leafasia Yasuo Chinzei Nobuo Ohta |
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| Affiliation: | (1) Department of Medical Zoology, Mie University School of Medicine, Tsu, Japan, JP;(2) Department of Medical Zoology, Nagoya City University Medical School, 1 Azakawasumi, Mizuhocho, Mizuhoku, Nagoya 467-8601, Japan e-mail: nohta@med.nagoya-cu.ac.jp Tel.: +81-52-8538184; Fax: +81-52-8420149, JP;(3) Department of Parasitology, Institute of Basic Medical Science, University of Tsukuba, Ibaraki, Japan, JP;(4) Department of Medical Zoology, Jichi Medical School, Tochigi, Japan, JP;(5) International Center of Medical Research, Kobe University School of Medicine, Kobe, Japan, JP;(6) Laboratory of Biology, Osaka Institute of Technology, Osaka, Japan, JP;(7) Solomon Institute of Medical Training and Research, Honiara, Solomon Islands, |
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| Abstract: | We analyzed the relationships between levels of antibody specific for merozoite surface glycoprotein-1 (MSP1) of Plasmodium falciparum and clinical manifestations in humans. We prepared recombinant MSP1 proteins representing block 3 (M3), block 6 (M6), blocks 1–6 (M1/6), and block 17. When we divided the slide-positive individuals in Guadalcanal into symptomatic and asymptomatic groups, the former group showed lower IgG levels against M6 and block 17, but not against M3, than did the asymptomatic group (P < 0.01). The possibility of nonspecific suppression was unlikely, given that the levels of antibody against poliomyelitis virus observed in the two groups were almost the same. Among the IgG subclasses tested, production of cytophilic IgG3 seemed to be dominant. When we analyzed epitopes recognized by antibodies against block 17, a peptide (SSSNFLGIS) was preferentially recognized by sera from asymptomatic individuals. These results suggest that clinical symptoms occurring during falciparum malaria seem to be associated with the development of levels of antibody against particular epitopes on MSP1, which is under the control of an immunoregulatory mechanism. Received: 1 October 1999 / Accepted: 21 October 1999 |
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