HPLC测定大鼠血浆中钩藤碱的浓度

目的建立HPLC测定大鼠血浆中钩藤碱浓度的方法。方法色谱柱为ZORBAX SB C18柱（150mm×4.6 mm,5 m）。以甲醇-水（80：20）（含0.0025 mol.L-1三乙胺,冰乙酸调pH至7.5）为流动相,检测波长为254 nm,流速为1.0 ml/min,柱温为30℃,进样量为20 l。结果钩藤碱与内源性杂质有较好分离度,钩藤碱在0.39~12.50g/ml浓度范围内呈良好线性关系;最低检测浓度为0.39 g/ml;回收率大于95%;日内和日间RSD均小于7%。结论此方法准确可靠,可用于测定钩藤碱的血药浓度。

Determination of the plasma Rhynchophylline level in rats by HPLC

Objective To establish the method to determine the plasma level ofRhynchophylline （RHY） in rats by HPLC. Methods The plasma samples were chromatographed on ZORBAX SB C1s column （150mm×4.6 mm,5 m） with a mixture of methanol and water （80： 20） as the mobile phase including 0.0025 mol/L triethylamine through glacial acetic acid to adjust pH 7.5. The detector wavelength was 254 nm. The flow-rate was 1.0 ml/min and the column temperature was set at 30℃. The injection volume was 20 μl. Results The linearity was obtained within the range of RHY concentrations from 0.39 to 12.50 μg/ml. The minimal inspect level was 0.39 μg/ml. The average recovery was more than 95%. Both of the inter- and intraoday RSD were less than 7%. Conclusion This method is stable, simple, rapid and accurate and applicable for the determination of plasma RHY level.