细胞色素C、Smac与胎膜早破相关性的研究

目的探讨细胞色素c（cytochrome c,Cyt-c）及半胱氨酸天冬氨酸蛋白水解酶激活剂（second mitochondria-derived activator of caspase,Smac）在胎膜早破中的表达以及与胎膜早破的关系。方法胎膜早破孕妇40例,20例正常孕妇（无妊娠合并症、并发症）作为对照组,均取胎膜破口处组织（2cm×1.5cm×0.5cm）,常规石蜡包埋切片,采用免疫组织化学方法测定细胞色素C、Smac在胎膜组织中的表达。结果①两组病例的胎膜组织均见Cyt-c、Smac的表达;③Cyt-c实验组平均光密度值为0.18±0.04,对照组为0.10±0.02,差异有显著性（〈0.05）;Smac实验组平均光密度值为0.18±0.03,对照组为0.10±0.03,差异有显著性（〈0.05）;③胎膜早破组Cyt-c、Smac的表达呈正相关（r=0.34,〈0.05）。结论胎膜早破中Cyt-c、Smac高表达,推测胎膜早破的发生与细胞凋亡有关。

Relative research among Cyt-c, Smac and premature rupture of membrane

Objective To investigate the expressions of Cyt-c and Smac and its role in premature rupture of membranes. Methods Pregnant women were classified as two groups, 40 cases with PROM （premature rupture of membrane） as observed group and 20 cases （no pregnancy complications） without PROM as controls group Samples （2cm×1.5cm×0.5cm） were taken from the rupture site of the membranes and paraff＇m embedded into sections both in two groups. The expression of Cyt-c and Smac in two groups were determined by immunohistochemistry. Results （1） Positive expressions of Cyt-c and Smac were observed in the fetal membranes both in two groups. （2） The mean density of Cyt-c expression in observed and control groups were 0.18±0.04 and 0.10±0.02 （P〈0.05）, mean density of Smac expression were 0.18±0.30 and 0.10±0.30 （P〈0.05）, respectively. （_3）Meanwhile, Cyt-c and Smac had positive correlation in PROM group. （r=0.34, P〈0.05） Conclusion Premature rupture of membranes occurred with high expressions of Cyt-c and Smac, and this may suggest that the occurrence of premature rupture of membranes was associated with apoptosis.