参芪扶正注射液对内毒素致急性肺损伤大鼠肺组织Fractalkine表达的影响

目的 动态观察趋化因子Fractalkine（FKN）在内毒素（LPS）所致急性肺损伤（ALI）大鼠肺组织内表达水平的变化，和参芪扶正注射液对其的影响。方法 经尾静脉注射LPS（4mg／kg）建立AL1大鼠模型。将42只大鼠随机分为7组，每组6只：正常对照组、模型1、2、4h 3个时相组和参芪扶正注射液1、2、4h3个时相组。采用酶联免疫吸附测定法（ELISA）、逆转录多聚酶链反应（RT-PCR）等方法。观察ALl大鼠模型肺组织病理学改变、肺湿干重比率（W／D）及血清肿瘤坏死细胞因子α（TNF-α）变化，检测肺组织FKN mRNA的表达，同时观察参芪扶正注射液对上述指标的影响。结果 （1）模型1、2、4h组肺组织病理改变明显，肺损伤程度重，以2h组最为显著，参芪扶正注射液能减轻ALI大鼠肺组织病理变化。（2）模型3个时相组肺W／D均明显增加，参芪扶正注射液能降低各时相组肺W／D值。（3）模型3个时相组血清TNF-α均明显增高，于2h点达到最高值，参芪扶正注射液能显著降低各时点血清TNF-α水平（P〈0．05）。（4）正常大鼠肺组织有FKNmRNA的表达，模型3个时相组肺组织FKNmRNA表达较正常组明显增加，在2h时点达最高值，参芪扶正注射液能降低ALI大鼠肺组织FKNmRNA的表达（P〈0．05）。FKN mRNA的表达与血清TNF-α水平呈正相关。结论 早期使用参芪扶正注射液能改善ALI肺组织病理改变。减轻肺水肿，降低血清TNF-α水平，下调肺组织FKNmRNA的表达，对内毒素致急性肺损伤实验大鼠具有保护作用。

Effect of Shenqi Fuzheng Injection on Fractalkine Expression in Lung Tissue of Rats with Lipopolysaccharide-induced Acute Lung Injury

OBJECTIVE: To observe dynamically the Fractalkine (FKN) expression in lung tissue of rats with lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the effect of Shenqi Fuzheng Injection (SFI) on it. METHODS: Rat model of ALI was established by intravenous injection of 4 mg/kg of LPS. Forty-two Wistar rats were randomly divided into 7 groups, the normal group, the model group and the SFI group, the latter two were separated respectively into three time phases (the 1 h, 2 h and 4 h after modeling ) groups, 6 rats in each group. Pathological changes and wet/dry weight ratio (W/D) of lung were observed, serum TNF-alpha and FKN mRNA expression in the lung tissue were examined by ELISA and RT-PCR respectively. RESULTS: Severe pathological changes of lung presented in the model groups of all three time phases with a higher W/D ratio, as well as increased serum TNF-alpha level and FKN mRNA expression in lung tissue. The peak of abnormality of serum TNF-alpha level and FKN mRNA expression was shown in the 2 h time phase group. All the above-mentioned abnormal changes were alleviated after treatment in the SFI group (P<0.05). In addition, the level of FKN mRNA expression was found to be positively correlated to the serum TNF-alpha concentration. CONCLUSION: SFI treatment in early stage could relieve the pathological changes and edema in lung tissue, decrease serum TNF-alpha and down-regulate FKN mRNA expression, playing a protective role in LPS-induced ALI rats.