环磷酰胺对雄性大鼠生精功能的影响

目的 观察环磷酰胺对大鼠生精功能的影响.方法 8周龄成年雄性sD大鼠,分6组,每组16只.前5组为实验组,给药方法分别为每日10、20、40、80、100 mg/kg,另1组为对照组.胃管给药2周和4周后,处死大鼠,取附睾精子行CASA检查;取睾丸组织苏木素.伊红(HE)染色分析睾丸曲细精管结构变化,TUNEL法检测睾丸曲细精管细胞凋亡情况.留取血清标本采用电化学发光法检测性激索水平.结果 环磷酰胺每日40 mg/kg喂养4周,大鼠存活率93.8%,精子数量明显减少(29.36±8.64)X 106个/ml,精子活力降低(22.25±2.03)%,睾丸生精上皮细胞明显损伤变性(58.1±1.2)%,血清睾酮水平下降(0.149±0.020)μg/L.这些指标的变化与环磷酰胺给药剂量及时间旱负相关(P<0.05).结论 通过环磷酰胺诱导可以成功建立大鼠少精/无精症动物模型,环磷酰胺主要通过坏死和凋亡两种途径导致睾丸曲细精管结构变化,导致生精细胞减少并最终引起少精/无精症.

The effect of cyclophosphamide on spermatogenic functions in rats

Objective To evaluate the effect of cyclophosphamide on spermatogenic function in rats. Methods Ninty-six male adult SD rats (8 weeks old) were equally divided into 6 groups. Different in A-E groups. The last group served as control. 14-28 days after the administration of the drug via the gas-tric tube, the rats were sacrificed, and their sperms taken from the epididymis were examimined with the techniques of CASA. The structural changes in the convoluted seminiferous tubules of their testis were pathologically studied by HE staining, and apoptosis of the cells of these tubules was detected by the TUNEL method. The sexual hormone levels in the serum of these animals were also tested. Results After sperms were reduced by (29.36±8.64) x 106/ml, sperm viability was decreased by(22.25±2.03)%, the testis germ cells were obviously damaged and degenerated, and levels of sexual hormone was declined to (0. 149±0.020) μg/L. All these changes were negatively correlated with the does and time of cyclo-phosphamide(P < 0. 05 ). Conclusion Models of oligoazoospermia/azoospermia could successfully estab-hshed in rats following the administration of cyclophosphamide.