长春碱分子印迹聚合物中模板分子的抽提

目的 研究从长春碱(VLB)分子印迹聚合物中抽提模板分子的合适条件.方法 将制备的VLB分子印迹聚合物填充于固相萃取小柱中,依次用不同比例的甲醇-冰醋酸溶剂洗脱,测定洗脱液中模板分子VLB的含量,计算回收率.结果 采用分子印迹聚合物最常用的抽提溶剂甲醇-冰醋酸(9∶1,v/v)抽提聚合物,模板分子VLB的回收率为91.71%,但由于VLB洗脱不完全,存在"模板渗漏";而用甲醇-冰醋酸(6∶4,v/v)为溶剂抽提聚合物,不仅能减少抽提次数及溶剂用量,而且模板分子VLB的回收率能提高到98.03%.抽提后的聚合物通过氢键等非共价键作用能特异性地吸附模板分子VLB,而对VLB的结构类似物长春新碱没有选择性.结论 用甲醇-冰醋酸(6∶4,v/v为溶剂抽提聚合物,模板分子洗脱完全,聚合物性质稳定,对模板分子VLB表现出高度的选择性和特殊的识别性能.

Removal of the template molecules from vinblastine-imprinted polymer

Objective To investigate the optimal condition for complete removal of the template molecules from vinblastine(VLB)-imprinted polymer.Method The prepared polymers were packed into the cartridges of solid-phase extraction column and washed by methanol-glacial acetic acid mixture with different proportions.The contents and recoveries of VLB in the effluents were determined.Results Polymer extraction with methanol-glacial acetic acid(9:1,V/V)resulted in VLB recovery of 91.73%,but template bleeding was observed because of incomplete VLB removal.Using methanol-glacial acetic acid(6:4,V/V)as the extraction solvent,the recovery of VLB reached 98.03% with less solvents and extract times.The polymers could selectively adsorb VLB through non-covalent interactions and still exhibited strong affinity for the template molecule but not for the structural analogue vincristine after extraction with methanol-glacial acetic acid(6:4,V/V).Conclusion Methanol-glacial acetic acid(6:4,V/V)is an ideal extract solvent for complete template molecule removal from the polymers,and the processed polymers possess stable capacity of specific recognition and selectivity to the template.