护骨素基因A163G多态性与北京地区老年人群骨密度的关系

背景：护骨素基因是骨质疏松症的候选基因,其基因多态性存在种族差异。目的：探讨北京地区老年人群中护骨素基因启动子区域A163G位点多态性与骨密度的关系。 方法：选取307名老年受试者,采用双能X射线骨密度吸收仪测定股骨近端、腰椎及前臂骨密度,取其外周血提取DNA,采用多聚酶链反应-限制性片段长度多态性方法测定护骨素基因A163G多态性,并进行基因测序。采用单因素方差分析比较不同基因型对应的骨密度变化,逐步回归法分析骨密度的相关因素。结果与结论：在绝经后老年女性受试者中,腰椎、股骨Inter区,AA型骨密度高于AG或GG型,老年男性受试者中,各基因型所对应的骨密度,没有明显差异。提示护骨素基因启动区域A613G多态性与绝经后老年女性骨密度有一定关联。

Association between A163G polymorphism of the osteoprotegerin gene and bone mineral density in the Chinese elderly from Beijing Area

BACKGROUND: Osteoprotegerin gene is the candidate gene of osteoporosis, and the ethnic divergence of gene polymorphisms exists. OBJECTIVE: To investigate the association between A163G polymorphism of the osteoprotegerin gene and bone mineral density in the elderly in Beijing. METHODS: A total of 307 elderly were included in this study. The bone mineral density in proximal femur, lumbar vertebra and forearm was determined by dual energy X-ray absorptiometry and DNA was extracted from peripheral blood. The A163G polymorphism of the osteoprotegerin gene was assessed by polymerase chain reaction-restriction fragment length polymorphism analysis and gene sequencing was performed. Differences in bone mineral density among different genotypes were compared using one-way analysis of variance. Bone mineral density-related factors were analyzed using multiple stepwise linear regression analysis. RESULTS AND CONCLUSION: In elderly women, the AA genotype of the A163G was associated with significantly lower bone mineral density at lumbar and femoral inter area; while in elderly men, no association was found between the A163G polymorphisms and bone mineral density. These findings suggest that A163G genotypes in the osteoprotegerin gene promoter are associated with bone mineral density in elderly women.