| 绵羊附睾种布鲁氏菌019株外膜蛋白基因序列比较研究 |
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| 引用本文： | 王远志,陈创夫,崔步云,曹旭东,张辉.绵羊附睾种布鲁氏菌019株外膜蛋白基因序列比较研究[J].疾病监测,2010,25(9):737-740. |
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| 作者姓名： | 王远志 陈创夫 崔步云 曹旭东 张辉 |
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| 作者单位： | 1.石河子大学医学院/新疆地方与民族高发病教育部重点实验室,新疆 石河子 832003; |
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| 基金项目： | 国家自然科学基金（No.30800813和31060334）;石河子大学高层次人才科研启动项目（No.RCZX200828）;兵团博士资金（ No.2009JC15 ）;973国家攻关项目（No.2010CB530200） |
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| 摘 要： | 目的证实新疆分离绵羊附睾种布鲁氏菌019株与参考菌株的差异性。方法采用PCR扩增绵羊附睾种布鲁氏菌019株外膜蛋白的Omp31、Omp25、Omp22、Omp2a、Omp2b基因,并进行序列比较分析。结果绵羊附睾种布鲁氏菌019株的5种外膜蛋白基因序列与绵羊种布鲁氏菌参考株在核苷酸水平和氨基酸水平上均存在差异。结论绵羊附睾种布鲁氏菌019株是独特的新疆地方株。
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| 关 键 词： | 绵羊附睾种布鲁氏菌019株 外膜蛋白基因 PCR 序列 |
| 收稿时间： | 2009-11-02 |
Comparative study on omp gene sequences of Brucella ovis 019 strain |
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| WANG Yuan-zhi
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CHEN Chuang-fu
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CUI Bu-yun
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CAO Xu-dong
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ZHANG Hui.Comparative study on omp gene sequences of Brucella ovis 019 strain[J].Disease Surveillance,2010,25(9):737-740. |
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| Authors: | WANG Yuan-zhi CHEN Chuang-fu CUI Bu-yun CAO Xu-dong ZHANG Hui |
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| Institution: | 1.The Medical College of Shihezi University, Shihezi 832003, Xinjiang, China |
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| Abstract: | Objective To prove the difference between Brucella ovis 019 strain isolated in Xinjiang and reference strain. Methods The Omp31、Omp25、Omp22、Omp2a、Omp2b gene sequences of Brucella ovis 019 strain were analyzed by polymerase chain reaction and DNA sequencing. Results There was significant difference in either DNA or amino level between Brucella ovis 019 strain and 63/290 reference strain; Conclusion Brucella ovis 019 strain is an unique local strain in Xinjiang. |
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| Keywords: | Brucella ovis 019 strain Omp gene polymerase chain reaction sequence |
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