ICR小鼠人工感染泰泽氏菌的组织病变观察

20只ICR小鼠经尾静脉途径人工感染泰泽氏菌。感染小鼠健康活泼,与对照小鼠无区别。感染后4-7天,lO只经醋酸可的松激发的小鼠其肝脏均表现出轻重不等的散在、灶性病变以至弥漫性灶性坏死,Gierasa染色镜检可见数量不等、典型的泰泽氏菌,细菌培养阴性。10只未经激发小鼠和5只对照小鼠均未见有病变,镜检也未查到泰泽氏菌。上述试验结果表明,泰泽氏菌在ICR小鼠体内的繁殖和形成肝脏病变与机体的免疫状态有密切关系。

Histopathologic manifestation in ICR mice experimentally infected with Clostridium Piliformis

20 ICR mice were inoculated withClostridium piliformis via tail vein. Clinical signs of Tyzzer's disease in infected micewere not observed. Gr oss lesion of liver with varying severity in 10 mice treated withcortisone acet ate was observed 4 to 7 days post-infection. Typical Clostridium piliformison s tamp preparation of postmortem liver were seen in a “pick-up-stick” arrangemen t within or beyond hepatocytes with Giemsastain. Bacterial culture on artificia l media was negative. Meanwhile no gross lesions andClostridium piliformis were identified both in 5 sham control mice and 10 no cortisonetreatment mice, The above results suggest that inducing gross lesion of liver andClostridium pilifo rmis propagation are associated with immunological state ofexperimental animals .