水芹水提物在鸭原代肝细胞培养中对DHBV抑制作用的初步研究

 目的：应用鸭乙型肝炎病毒（DHBV）体外肝细胞培养模型，研究中草药水芹（SQ）抗乙型肝炎病毒（HBV）的作用。方法：制取北京雏鸭原代肝细胞（50万／ml／孔／24孔板）于37℃，CO₂培养24h存活后，除对照组外，给药组每天换药1次，连续加药5d并测定DNA的含量。结果：①在肝细胞毒性实验中，SQ对鸭肝细胞无毒性，半数有毒浓度（CD₅₀）大于10000μg·ml^－1。②在DHBV感染鸭的原代肝细胞培养中能够保护肝细胞，连续给药培养7d，肝细胞的贴壁状态、形态和存活量均优于对照组。③对DHBV DNA有明显的抑制作用，其抑制率（2500μg·ml^－1）为64％，半数有效浓度（ED₅₀）为1120．8μg·ml^－1，选择指数（SI）大于9。结论：实验结果提示，SQ对DHBV有一定的抑制作用。

Inhibition of Shuiqin on DHBV in vitro

OBJECTIVE: Shui Qin (Oenanthe javanica, SQ) is a Chinese herbal drug to cure hepatitis popular. We use SQ to study its effect on hepatitis modesl in animals. METHOD: Duck hepatitis B virus (DHBV) infected duck primary hepatocytes culture in tube established By Hong-shan chen was taken to detect the inhibition of SQ on DHBV. RESULTS: SQ potentially inhibited DHBV DNA. The maximal inhibition was 64% and ED₅₀was 1 120.8 μg·ml^-1 which was much less than its CD₅₀(1 000 μg·ml^-1)、SI was more than 9.0. CONCLUSION: SQ is a hopeful drug for control DHBV.