CXCR4在大肠腺癌中的表达及临床意义

The expression and clinical significance of CXCR4 in colorectal adenocarcinoma

Objective To evaluate the role and clinical significance of C-X-C chemokine receptor 4 （CXCR4） in development,infiltration and metastasis of colorectal carcinoma. Methods The expressions of CXCR4 protein were detected by SP immunohistochemical（IHC） method in 60 cases of eoloreetai adenocarci- noma, 23 cases of eolorectal adenoma and 22 cases of normal colorectal mucosa. The expressions of CXCR4 mRNA were detected by RT-PCR method in 30 cases of colorectal adenocarcinoma, 15 cases of normal col- orectal mucosa. Results IHC results showed that the expressional quantity of CXCR4 protein in the colorectal adenocarcinoma was obviously higher than that in the colorectal canalicular adenoma and normal colorectal mucosa , and the differences all had statistical significance （P all〈 0.05）.The expressional quantity of CXCR4 protein in the colorectal canalicular adenoma was obviously higher than that in the normal colorectal mucosa, and the difference had statistical significance （P〈 0.01 ）.The results of RT-PCR showed that the expressional quantity of CXCR4 mRNA in the colorectal adenocarcinoma was obviously higher than that in the normal col- orectal mucosa,and the difference had statistical significance （P〈 0.01 ）.The positive expressions of CXCR4 protein and mRNA were closely correlated with the depth of tumor infiltration and metastasis of lymph node （P all〈 0.05）, but they were not related to gender and age of patients and differentiation of cancer （P all〉 0.05）. There was positive correlation between the expression of CXCR4 and CXCR4 mRNA （r= 0.588, P〈 0.01）. Conclusion High expression of CXCR4 in colorectal adenocarcinoma may play an important role in tumori-genesis progression, infiltration and metastasis of colorectal adenocarcinoma.