高效液相色谱分析虚拟仿真教学实施及效果评价

目的 研究传统教学结合虚拟仿真教学在高效液相色谱法中进行药物含量分析的教学效果。方法 以2016级两个平行班的学生为研究对象，分为试验组（44名）和对照组（38名）。以高效液相色谱法分析甲硝唑注射液含量及其方法学研究为例，试验组采用传统线下教学结合虚拟仿真教学，对照组采用传统线下教学。通过比较两组期中、期末考试成绩来评价两组教学效果。用SPSS 22.0软件进行独立样本t检验。结果 期中测试结果表明试验组在实际操作考核方面优于对照组（17.98±6.75） vs. （14.03±5.92）]，差异有统计学意义（P < 0.05），而在理论、汇报及报告成绩方面两组差异无统计学意义。两组期末考试成绩各方面差异都无统计学意义。结论 采用传统线下教学结合虚拟仿真训练，可提高学生的实际操作能力，有助于提高药物含量分析实验的教学效果。通过本项目的训练，学生掌握了高效液相色谱法操作、药物制剂的含量测定、分析方法的验证、数据处理及分析报告的撰写等能力，具备了一定的实际分析工作能力。

Implementation and effect evaluation of virtual simulation teaching of high performance liquid chromatography

Objective To study the teaching effect of traditional teaching combined with virtual simulation teaching in pharmaceutical content analysis using high performance liquid chromatography (HPLC).Methods The students in two parallel classes of grade 2016 were divided into experimental group (44 students) and control group (38 students). Taking the content analysis and its related analytical methodology of Metronidazole by HPLC as an example, the experimental group adopted the traditional offline teaching combines with virtual simulation teaching, and the control group adopted the traditional offline teaching. The teaching effects of the two groups were compared. SPSS 22.0 was used to conduct independent-samples t-test.Results According to the mid-term test results, the experimental group was superior to the control group in practical operation assessment (17.98±6.75) vs. (14.03±5.92)], and the difference was statistically significant (P < 0.05). But there were no differences between the two groups in the scores of theory, discussion report and presentation. There was no significant difference in the final exam scores between the two groups.Conclusion The ability of practical training and the teaching effect can be improved by the teaching method of traditional offline teaching combined with virtual simulation. Through the training of this project, students have mastered the operation of HPLC, the content determination of pharmaceutical preparations, the verification of analytical methods, data processing, and the writing of analytical reports, and have certain practical analysis ability.