人参总皂甙对K562细胞增殖抑制与诱导分化的实验研究

采用细胞体外培养，流式细胞术、形态学观察、组化与免疫细胞化学等方法，研究人参总皂甙（ＴＳＰＧ）对Ｋ＿６５２细胞增殖与分化的影响。结果表明：ＴＳＰＧ对Ｋ＿５６２细胞增殖具有明显的抑制作用，并能阻止Ｋ６５２细胞由Ｇ０／Ｇ１期向Ｇ２／Ｍ＋Ｓ期过渡：经２００μｇ／ｍｌ的ＴＳＰＧ诱导后，Ｋ５６２细胞形态趋向成熟分化；联苯胺染色、糖原染色和ＨＩＲ２．ＣＤｗ４１阳性表达细胞数显著提高，且阳性强度增加。推测ＴＳＰＧ是能抑制白血病细胞增殖并诱导其分化的天然诱导剂，但对其机理有待进一步探讨。

Experimental study on the effect of proliferation and differentiation ofK562 cell treated with TSPG

By using cell culture ex.vivo, flow cytometry (FCM), morphological observation, cytochemistry and immunocytochemistry, the effects of Total Saponins of Panax Ginseng (TSPG) on proliferation and differentiation of K562 cells were studied. The results indicated that TBPG. could markedly inhibit the proliferation of K562 cells , block K562 cells entering the active proliferative phase (G2/M S), and promote K562 cells to differentiate toward the mature blood cell. Our experiment also showed that after induced by TSPG, the ratio of positive K562 cells of expressing HIR2 CDw4t, glycogen, benzidine stain is obviously higher than that of control,Combining with our previous reasearcb works, we infer that TSPG may be one of the natural inducers which can not only enhance normal hematopoiesis, but also inhibit proliferation of such cancer cells as leukemia cells, and induce differentiation of these cells. The mechanism should be continually studied.