| 巢式多聚酶链反应快速检测胎儿性别 |
| |
| 引用本文： | 柳青 陈宏础. 巢式多聚酶链反应快速检测胎儿性别[J]. 重庆医科大学学报, 1999, 24(1): 41-42 |
| |
| 作者姓名： | 柳青 陈宏础 |
| |
| 作者单位： | Department of core laboratory，college of clinical medicine |
| |
| 摘 要： | 为建立一种非侵入性的对Ｘ性连锁遗传性疾病胎儿的早期产前性别鉴定方法，我们采用了巢式ＰＣＲ方法来扩增孕妇外周血中男性胎儿的Ｙ染色体特异序列，并与新生儿出生性别相对照。４４例孕妇中２２例分娩男性婴儿．其中１９例扩增出Ｙ特异片段，阳性检出率为８６．４％（１９／２２）．其余２２例分娩女性婴儿，其中无一例出现Ｙ特异扩增带，结果提示用巢式ＰＣＲ方法可快速判断胎儿性别，使临床上无伤性产前检查Ｘ性连锁遗传性疾病的胎儿性别成为可能。
|
| 关 键 词： | 巢式多聚酶链反应，胎儿性别鉴定 |
Quick fetal sex determination by nested polymerase chain reaction |
| |
| Liu Qing,et al.. Quick fetal sex determination by nested polymerase chain reaction[J]. Journal of Chongqing Medical University, 1999, 24(1): 41-42 |
| |
| Authors: | Liu Qing et al. |
| |
| Abstract: | To establish a non-invasive method for early prenatal sex determination of the fetus carrying sex- linkedgenetic disorder. The fetal specific fragment of Y chromosome was amplified by nested polymerase chain reactionfrom the preipheral maternal blood, and the results were compared with new born baby gender after parturition.The Y-specific sequence was identfied in 19 of 22 mothers given the male babies, positive rate was86.4%(19/22), but unidentified in 22 mothers given the female babies. The result show that fetal sex deterinationby nested PCR will be (suitable) for clinical prenatal diagnosis of sex- linked genetic disorders. |
| |
| Keywords: | Nested polymerase chain reaction Fetal sex determination |
| 本文献已被 CNKI 维普 等数据库收录！ |
