成纤维细胞生长因子及受体在肾小管间质纤维化过程中的表达

利用免疫组织化学及原位杂交技术观察30例肾间质纤维化模型大鼠，探讨碱性成纤维细胞生长因子（bFG）及受体（FGFR－1）与肾小管间质纤维化的关系。结果显示大鼠模型中损伤的肾小管上皮过度表达bFGF、FGFR－1，且合成Ⅲ型胶元；肾间质细胞广泛表达bFGF和FGFR－1，而且此表达程度和间质细胞核增殖及小管间质Ⅲ型胶元沉积程度一致．本实验证实肾小管损伤刺激bFGF、FGFR－1的分泌，提示bFGF可能通过自分泌和（或）旁分泌机制促进肾小管上皮的再生修复、间质细胞的增殖及Ⅲ型胶元的合成，从而促进肾小管间质纤维化的形成。

The expression of basic fibroblast growth factor(bFGF)and FGFR-1 in renal tubulointerstitial fibrosis

The relationship between the expression of bFGF, FGFR-1 and renal tubulointerstitial fibrosis wasinvestigated by using immunohistochemistry and in situ hybridization techniques in thirty rat renaltubulointerstitial fibrosis models. The results showed that injured tubular cells excessively synthesized bFGF,FGFR- 1 and collagen IH; in addition, interstitial celIs widely expressed bFGF and FGFR- 1, their degree ofexpression accorded with PCNA expression in these cells and with collagen III deposition in thetubulointerstitium.It suggested that injury to tubular cells enhenced the secretion of bFGF and FGFR- 1.bFGFprobably promoted the regeneration of injured tubular celIs,the proliferation of interstitial cells and the synthzsisof collagen III through autocrine and/or paracrine mechanism, and thus accelerated the fibrosis of the renalinterstitium.