The in vitro biosynthesis of steroids by the gonad of the cuttlefish (Sepia officinalis L.) |
| |
Authors: | S Carreau M Drosdowsky |
| |
Institution: | Département de Biochimie, C.H.U. Côte de Nacre, 14000 Caen, France |
| |
Abstract: | In vitro steroid biosynthesis in the male and female gonads of Sepia officinalis was investigated. Using tritium- and 14C-labeled precursors, seven enzyme systems were demonstrated: a C20,22-lyase (cholesterol→pregnenolone), a 3β-hydroxysteroid dehydrogenase-Δ5?4-isomerase complex (pregnenolone → progesterone; 17α-hydroxypregnenolone → 17α-hydroxyprogesterone; dehydroepiandrosterone → androstenedione), a 17α-hydroxylase (pregnenolone → 17α-hydroxypregnenolone; progesterone → 17α-hydroxyprogesterone), a C17,20-lyase (17α-hydroxypregnenolone → dehydroepiandrosterone), a 17β-hydroxysteroid dehydrogenase (dehydroepiandrosterone → androstenediol; androstenedione → testosterone; estrone→estradiol-17β), a 20α-ol dehydrogenase (progesterone→20α-dihydroprogesterone), and a 20β-ol dehydrogenase (progesterone→20β-dihydroprogesterone). The yields were rather low (0.01–3%), except when pregnenolone was used (8%). Radioimmunoassay techniques indicate the presence of testosterone in the plasma of the cuttlefish but the absence of estrogens. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|