| 人脑神经干细胞的分离培养及鉴定的研究 |
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| 引用本文： | 汪泱,邓志锋,赖贤良,王共先,黄学明,付斌,刘伟鹏.人脑神经干细胞的分离培养及鉴定的研究[J].江西医学检验,2004,22(1):11-12,20. |
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| 作者姓名： | 汪泱 邓志锋 赖贤良 王共先 黄学明 付斌 刘伟鹏 |
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| 作者单位： | 江西医学院第一附属医院泌尿外科研究所，江西医学院第二附属医院神经外科，江西医学院第二附属医院神经外科，江西医学院第一附属医院泌尿外科研究所，江西医学院第一附属医院泌尿外科研究所，江西医学院第一附属医院泌尿外科研究所，江西医学院第一附属医院泌尿外科研究所 330006南昌，330006南昌，330006南昌，330006南昌，330006南昌 |
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| 基金项目： | 国家自然科学基金资助项目(30160084)江西省自然科学基金项目(0240047) |
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| 摘 要： | 目的探讨分离培养人脑神经干细胞的方法及条件。方法用胰蛋白酶消化法从人胚脑中分离单个细胞,用无血清培养基进行体外培养,bFGF和EGF刺激细胞生长,用血清诱导其分化;采用免疫荧光细胞化学染色方法检测培养的细胞神经巢蛋白(Nestin)抗原和分化后成熟神经细胞抗原神经元特异性烯醇化酶(NSE)的表达。结果从胚龄16周的新鲜人胚脑中成功分离出神经干细胞。该细胞可在体外培养条件下传代培养,并表达神经干细胞标志Nestin;分化后的细胞主要表达成熟神经细胞标志NSE。结论在体外培养条件下可从人胚脑中分离培养出神经干细胞。
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| 关 键 词： | 神经干细胞 人胚 细胞培养 |
| 文章编号： | 1008-0023(2004)01-0011-03 |
Study on isolation, cultivation and identification of neural stem cells from human embryonic brain |
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| WANG Yang,DENG Zhifeng,LAI Xianliang,et al..Study on isolation, cultivation and identification of neural stem cells from human embryonic brain[J].Jiangxi Journal of Medical Laboratory Sciences,2004,22(1):11-12,20. |
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| Authors: | WANG Yang DENG Zhifeng LAI Xianliang |
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| Institution: | WANG Yang,DENG Zhifeng,LAI Xianliang,et al.The First Affiliated Hospilal,Jiangxi Medical College,Nanchang330006,China |
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| Abstract: | Objective To explore the culture conditions of human neural stem cells from embryonic brain.Methods The cells were derived from the sixteen-week embryonic brain di gested by trypsin,and cultured in serum free medium with EGF and bFGF.The differention of cells was induced by culture with serum.Nestin antigen and NSE were detected by immunofluo rescence test.Results Human neural stem cells from fresh embryonic brain at16-week were iso lated successfully,and passaged continuously.The cells expressed Nestin antigen which was the marker for the neural stem cells,and the cells induced by serum expressed(NSE)neuron specific enolase markers for neuron.Conclusion Human neural stem cells from embryonic brain can be isolated and cultured in vitro. |
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| Keywords: | Neural stem cell Human embryo Cell culture |
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