消癃通闭对大鼠前列腺组织碱性成纤细胞生长因子表达的影响

目的：了解中药消癃通闭对前列腺组织碱性成纤维细胞生长因子（ＢａｓｉｃＦｉｂｒｏｂｌａｓｔＧｒａｏｔｈＦａｃｔｏｒ，ｂＦＧＦ）表达的影响。方法：以去势Ｗｉｓｔａｒ大鼠，经皮下每天注射睾酮，同时给予中药消癃通闭药粉灌胃，２０ｄ时断髓处死。取相同部位前列腺腹叶，以免疫组化定量技术对前列腺组织ｂＦＧＦ进行测试。结果：消癃通闭高剂量组的ｂＦＧＦ表达明显低于睾酮组，Ｐ＜００１；并发现ｂＦＧＦ的表达主要位于腺上皮细胞的胞浆，而胞核及间质部分表达较少。结论：中药消癃通闭治疗前列腺增生的机理之一是该药明显抑制了前列腺组织中的ｂＦＧＦ的表达，进而抑制了前列腺细胞的生长

Effect of XLTB on Expression Level of bFGF in Rats Prostatic Tissue

Objective:To investigate the effect of Xiao Long Tong Bi(XLTB,a traditional Chinese herb medicine)on the expression level of bFGF in rat prostatic tissue.Methods:Subcutaneous injection of testosterone was given every day to castrated male Wistar rats,while XLTB was given by gavage for 20 days,then the rats were executed,the same parts of the prostate were taken out,in which the expression of bFGF were quantitatively assayed by immunohistochemical technique and computer assited image analysis.Results:The expression level of bFGF in rat prostate fed with high dose of XLTB is much lower than the testosterone group, P<0.01 ;and the expressions were predominantly located in the epithelial cytoplasm,seldom in muclei and stroma.Conclusion:The therapeutic mechanism of XLTB in the treatment of benign prostatic hyperplasia might be the inhibition of the expression of bFGF in prostatic tissue,subsequently inhibiting the growth of prostatic cells.