| 吡格列酮对大鼠骨骼肌细胞脂联素受体基因表达的影响 |
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| 引用本文： | 刘小莺,刘礼斌. 吡格列酮对大鼠骨骼肌细胞脂联素受体基因表达的影响[J]. 标记免疫分析与临床, 2007, 14(3): 160-163 |
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| 作者姓名： | 刘小莺 刘礼斌 |
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| 作者单位： | 福建医大附属协和医院内分泌研究所,福建,福州,350001;福建医大附属协和医院内分泌研究所,福建,福州,350001 |
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| 基金项目： | 福建省基金高校专项课题，青年基金立项课题 |
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| 摘 要： | 为研究吡格列酮对大鼠骨骼肌细胞脂联素受体表达的影响,应用体外原代培养骨骼肌细胞和SYBR Green Ⅰ实时定量PCR,观察不同浓度吡格列酮对鼠骨骼肌细胞脂联素受体表达水平的影响.结果表明,不同浓度的吡格列酮作用于大鼠骨骼肌细胞20h后对脂联素的受体R1表达没有显示出任何有意义的变化.大鼠骨骼肌细胞脂联素受体基因表达可能与胰岛素增敏剂噻唑烷二酮类吡格列酮无关.
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| 关 键 词： | 脂联素受体 脂联素 噻唑烷二酮类 SYBR Green Ⅰ |
| 文章编号： | 1006-1703（2007）03-0160-04 |
| 修稿时间： | 2006-12-11 |
Effects of Pioglitazone on Adiponectin Receptor Gene Expression in Rat Skeletal Muscle Cells |
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| LIU Xiao-ying,LIU Li-bin. Effects of Pioglitazone on Adiponectin Receptor Gene Expression in Rat Skeletal Muscle Cells[J]. Labeled Immunoassays and Clinical Medicine, 2007, 14(3): 160-163 |
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| Authors: | LIU Xiao-ying LIU Li-bin |
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| Affiliation: | Department of Endocrinology, Union Hospital of Fujian University, Fuzhou 350001,China |
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| Abstract: | In order to investigate the effects of thiazolidinediones on the expression of adiponectin receptors in rat skeletal muscle cells,the expression levels of adiponectin receptors in cultivated rat skeletal muscle cells in different thiazoidinediones concentration were determined by SYBR Green I quantitative real-time PCR.The results showed that the expression of adipoR1 mRNA in cultivated-rat skeletal cells had no significant changes after treatment with pioglitazone for 20 h.It suggested AdipoR1 gene expression in rat skeletal cells was not involved in the insulin sensitizing effects of pioglitazone thiazolidinediones. |
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