| 牙龈卟啉菌蛋白酶rgpA的基因克隆和序列分析 |
| |
| 引用本文： | 许静,李昂,苟建重,许援朝,饶国洲,谢红帼. 牙龈卟啉菌蛋白酶rgpA的基因克隆和序列分析[J]. 陕西医学杂志, 2006, 35(2): 163-165 |
| |
| 作者姓名： | 许静 李昂 苟建重 许援朝 饶国洲 谢红帼 |
| |
| 作者单位： | 西安交通大学口腔医学院,西安,710004 |
| |
| 基金项目： | 西安交通大学校科研和教改项目;陕西省西安市科技攻关项目 |
| |
| 摘 要： | 目的:扩增牙龈卟啉菌蛋白酶rgpA催化结构域(rgpAcd)的基因片断并作鉴定。方法:利用PCR方法,克隆牙龈卟啉菌rgpAcd基因,并将基因片断插入pMD18-TVector,通过限制性酶切和核苷酸序列分析鉴定。结果:克隆基因测序结果与GeneBank数据库中的序列一致。结论:成功克隆了牙龈卟啉菌rgpAcd的基因,为体外表达其活性蛋白奠定了基础。
|
| 关 键 词： | @牙龈卟啉菌 蛋白酶 克隆 |
| 收稿时间： | 2005-08-30 |
| 修稿时间： | 2005-08-30 |
Cloning and sequencing analysis of gingipain R (rgpA) of porphyromonas gingivalis |
| |
| Xu Jing, Li Ang, Gou Jianzhong,et al.. Cloning and sequencing analysis of gingipain R (rgpA) of porphyromonas gingivalis[J]. Shaanxi Medical Journal, 2006, 35(2): 163-165 |
| |
| Authors: | Xu Jing Li Ang Gou Jianzhong et al. |
| |
| Affiliation: | Xi'an 710004 |
| |
| Abstract: | Objective: To clone the catalytic domain of gingipain R (rgpAcd) of Porphyromonas gingivalis (Pg). Methods: The desired DNA fragment rgpAcd was obtained by PCR and was inserted into pMD18-T Vector. The double-stranded DNA of the positive clone was analyzed by restriction endonuclease mapping and DNA sequencing. Results: A 1476bp specific fragment was obtained and DNA sequencing showed that the fragment was consistent with those of the published. Conclusion: The rgpAcd of Pg was cloned successfully. The protein of rgpAcd will be obtained for further study. |
| |
| Keywords: | @Porphyromonas gingivalis Gingipain Clone |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
