小儿肺炎血清中粒细胞集落刺激因子水平测定及其临床意义的研究

应用抗粒细胞集落刺激因子（Ｇ－ＣＳＦ）单克隆抗体，采用酶联免疫吸附法对６１例小儿肺炎患儿血清中Ｇ－ＣＳＦ水平进行了测定，并观察了Ｇ－ＣＳＦ水平与患儿体温、白细胞总数、中性粒细胞比例，Ｃ－反应蛋白试验（ＣＲＰ）及治疗后Ｇ－ＣＳＦ水平的动态变化。结果显示：（１）６１例肺炎患儿Ｇ－ＣＳＦ阳性率为６２．２９％；（２）发烧组Ｇ－ＣＳＦ水平较不发烧组为高；（３）Ｇ－ＣＳＦ阳性者中其白细胞总数８９．４１％为正常，７３．６８％中性粒细胞比例增高，９４．４４％ＣＲＰ试验为阴性；（４）感染控制后Ｇ－ＣＳＦ迅速转为阴性。以上结果表明测定Ｇ－ＣＳＦ水平对小儿肺炎病因的诊断、合理应用抗生素提供了快速、灵敏、可靠的指标，具有重要的临床意义。

THE STUDY ON THE MEASUREMENT OF SERUM GRANULOCYTE COLONY-STIMULATING FACTOR LEVELS AND IT'S CLINICAL SIGNIFICANCE IN CHILDREN SUFERED FROM PNEUMONIA

Abstract: The Granulocyte colony-stimulating factor (G-CSF) levels in serum of 61 children suffered from pneumonia were measured by enzyme-linked immmunosorbent assay (ELISA) for G-CSF. The direct relationships between G-CSF levels and white blood cell count, absolute granulocyte numbers in blood, temperature of patients, C-reactive protein (CRP) were observed and the change of G-CSF levels after treated were also studied. The results showed:(1) 62. 29% of samples detected showed positive reaction for G-CSF; (2) The G-CSF levels were higher in the groups of fever than the groups of no fever; (3) 89. 41 % of white blood counts in cases examined were normal, 73, 68% of granulocytes were highter, 94. 44% of CRP tests showed negtive reaction in the cases of positive reaction for G-CSF; (4) The GCSF reactions become negtive immediately after the infectiion controlled. All these results suggest the detection of the levels of G-CSF in patients with pneumonia of children may apply a quick . sensitive and faithful mark for the cause of the disease, having an important elinical significance.