| 高糖对乳鼠心肌细胞凋亡及相关基因表达的影响 |
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| 引用本文： | 周〓祥,孔祥清,盛燕辉,杨〓荣,曹克将.高糖对乳鼠心肌细胞凋亡及相关基因表达的影响[J].南京医科大学学报,2010(4):437-441. |
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| 作者姓名： | 周〓祥 孔祥清 盛燕辉 杨〓荣 曹克将 |
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| 作者单位： | 南京医科大学第一附属医院心内科; |
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| 基金项目： | 江苏省“科教兴卫工程”医学重点学科基金;;江苏省高校自然科学基金;;江苏省卫生厅资助课题 |
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| 摘 要： | 目的:探讨高糖对乳鼠心肌细胞凋亡及相关基因表达的影响。方法:分离培养SD乳鼠心肌细胞,以20mmol/L终浓度的葡萄糖干预48h后,激光共聚焦显微镜、TUNEL染色和流式细胞术检测心肌细胞凋亡;荧光定量PCR检测高糖对凋亡相关基因P53、Pax3、Bmp4、Slug、Msx1表达的影响。结果:激光共聚焦显微镜观察可见高糖组心肌细胞凋亡明显增加;TUNEL检测心肌细胞凋亡指数,高糖组为(19.67±3.45)%,明显高于对照组(1.12±0.24)%(P<0.05);流式细胞术显示高糖组细胞凋亡率较对照组明显增加(P<0.05);荧光定量PCR表明高糖能上调P53、Msx1基因的表达,下调Pax3、Bmp4、Slug基因的表达。结论:高糖可能通过影响凋亡相关基因P53、Pax3、Bmp4、Slug、Msx1的表达而诱导心肌细胞凋亡。
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| 关 键 词： | 高糖 心肌细胞 凋亡 |
| 收稿时间： | 2009/12/19 0:00:00 |
Effects of high glucose on apoptosis and expression of apoptosis-related genes in neonatal rat cardiomyocytes |
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| ZHOU Xiang,KONG Xiang-qing,SHENG Yan-hui,YANG Rong and CAO Ke-jiang.Effects of high glucose on apoptosis and expression of apoptosis-related genes in neonatal rat cardiomyocytes[J].Acta Universitatis Medicinalis Nanjing,2010(4):437-441. |
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| Authors: | ZHOU Xiang KONG Xiang-qing SHENG Yan-hui YANG Rong and CAO Ke-jiang |
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| Institution: | Department of Cardiology/a>;the First Affiliated Hospital of NJMU/a>;Nanjing 210029/a>;China |
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| Abstract: | Objective:To investigate the effects of high glucose on apoptosis and expression of apoptosis-related genes in neonatal rat cardiomyocytes. Methods:Cultured cardiomyocytes were exposed to high glucose (20 mmol/L) for 48 h. Laser confocal microscopy,TUNEL assay and flow cytometry were utilized to detect cardiomyocyte apoptosis. Real-time PCR was performed to identify mRNA expression of apoptosis-related P53,Pax3,Bmp4,Slug and Msx1 genes in the glucose group and the control group. Results:Cardiomyocyte apopto... |
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| Keywords: | high glucose cardiomyocyte apoptosis |
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