脊髓急性损伤时NO、IL-8、IL-6的表达及意义

目的 :研究脊髓急性损害早期白细胞介素 -8(IL 8) ,白细胞介素 -6(IL 6) ,一氧化氮 (NO)及一氧化氮合酶 (NOS)的变化。方法 :76例脊髓急性损害病人 ,根据Franked分类 ,分完全截瘫 (FrankedA)组 ,不完全截瘫 (FrankedB ,C和D)组 ,以ELISA法 ,硝酸还原酶法分别测IL 8、IL 6、NO、NOS的值 ,以健康体检人员作对照。结果 :IL 8、IL 6的值完全截瘫者和不完全截瘫者都显著升高 (P <0 .0 5 ) ,NO、NOS的值完全截瘫者和不完全截瘫者都显著降低 (P <0 .0 5 )。结论 :脊髓急性损害早期IL 8、IL 6显著升高 ,NO、NOS显著降低 ,脊髓急性损害早期IL 8、IL 6、NO参与了脊髓继发性损害。

The Alternation of Nitric Oxide、Interlukin-6 and Intelukin-8 Level after Acute Spinal Cord Injury

Objective:To explore the functional mechanism of interlukin 8(IL 8),interlukin 6(IL 6),nitric oxide(NO) and nitric oxide synthase(NOS) at the early stage of acute spinal cord injury(ASCI).Methods:According to Franked methods,76 ASCI patients were divided into two groups:total paralysis group and partial paralysis group.The levels of IL 8,IL 6,NO and NOS were analyzed by an assay with nitric acid reductase and ELISA method.Results:Compared with the normal group,the IL 8,IL 6 level of partial and total paralysis group were elevated significantly( P<0.05 ),the NO and NOS level were markedly lower( P<0.05 ).Conclusions:The present data suggest that at the early stage of ASCI,the levels of IL 8 and IL 6 increased significantly,and the levels of NO and NOS decreased significantly.IL 8,IL 6 and NO might play certain role in the spinal cord secondary injury.